Antibody:	Anti-Hck
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural plate	Present
TS13: neural tube	Present
TS13: neural ectoderm	Present	Regionally restricted		Expression was detected in the neural tube when it was partially closed.

Structure	Level	Pattern	Image	Note
TS17: brain	Strong
TS17: future spinal cord	Strong

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Uniform
TS20: spinal cord	Present	Uniform
TS20: vagus ganglion	Present
TS20: vagal nerve trunk	Present	Regionally restricted		Expression was detected in nerve bundles around the cardiovascular system and respiratory tract.

Structure	Level	Pattern	Image	Note
TS23: choroid plexus	Present		1G
TS23: cerebellum	Present	Regionally restricted	1G	Expression was detected in the geminal trigone and external germinal layer.

Structure	Level	Pattern	Image	Note
TS25: ventral grey horn	Strong	Regionally restricted	1I	Expression was intense in motoneuron-like cells at the marginal zone of the ventral horn.

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		3A
TS19: future spinal cord	Present		3A
TS19: myotome	Present		3A

Structure	Level	Pattern	Image	Note
TS25: retina	Present		4D	Expression was in the inner part of the retina.
TS25: eyelid	Present		4E , 4D
TS25: lens	Present		4E , 4D
TS25: eyelid epithelium	Present	Regionally restricted	4D , 4E	Expression was detected in the epithelial layer of the fused part of the eyelid, including the epidermis on the outer eyelid surface and the conjunctiva on the inner eyelid surface.