Antibody:	Anti-Blk
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural plate	Present
TS13: neural tube	Present
TS13: neural ectoderm	Present	Regionally restricted		Expression was detected in the neural tube when it was partially closed.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted		Expression was dorsal.
TS17: midbrain	Present	Regionally restricted		Expression was dorsal.
TS17: brain	Strong
TS17: future spinal cord	Strong

Structure	Level	Pattern	Image	Note
TS19: forebrain	Present	Regionally restricted		Expression was dorsal.
TS19: midbrain	Present	Regionally restricted		Expression was dorsal.

Structure	Level	Pattern	Image	Note
TS20: cortical plate	Present
TS20: vagus ganglion	Present
TS20: vagal nerve trunk	Present	Regionally restricted		Expression was detected in nerve bundles around the cardiovascular system and respiratory tract.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	1D	Expression was detected in ventral spinal cord in motoneuron-like cells.
TS17: spinal nerve	Present		1D
TS17: dorsal root ganglion	Weak		1D

Structure	Level	Pattern	Image	Note
TS26: cochlear ganglion	Present		4F