Antibody:	anti-Connexin 43
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to Cy3

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch maxillary component endoderm	Present	Regionally restricted	2A	Expressed in the localized thickenings of the ventral epithelium of the developing maxilla.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component endoderm	Present	Regionally restricted	2B	Expressed in the localized thickenings of the dorsal epithelium of the developing mandible.
TS17: 1st branchial arch mandibular component mesenchyme	Present	Regionally restricted	2B	Expressed in the mesenchyme around the midline of the fusing mandibular processes.

Structure	Level	Pattern	Image	Note
TS19: mandibular process mesenchyme	Present	Regionally restricted	2C	Expressed in the odontogenic mesenchyme of the mandibular processes.

Structure	Level	Pattern	Image	Note
TS20: mandibular process mesenchyme	Present	Regionally restricted	2D	Expressed in the condensing mesenchyme adjacent to the invaginating odontogenic epithelium.

Structure	Level	Pattern	Image	Note
TS21: enamel organ	Present		2E	Expressed in the enamel organ of the bud-stage molar tooth germs.

Structure	Level	Pattern	Image	Note
TS23: tooth epithelium	Strong	Regionally restricted	2F	Expressed in the epithelium immediately adjacent to the dental papilla.
TS23: enamel organ	Present	Regionally restricted	2F	Expressed in the lateral aspect of the enamel organ. Not expressed in the medial portion.
TS23: dental papilla	Absent		2F

Structure	Level	Pattern	Image	Note
TS27: enamel organ	Strong	Regionally restricted	2H , 2G	Expressed in the secretory ameloblasts.
TS27: stratum intermedium	Strong		2G , 2H
TS27: stellate reticulum	Present		2G , 2H	Weaker expression.

Structure	Level	Pattern	Image	Note
TS28: incisor enamel organ	Strong	Punctate	2I	Expressed in the secretory ameloblasts.
TS28: stratum intermedium	Present		2I	Weaker expression.

Structure	Level	Pattern	Image	Note
TS28: incisor enamel organ	Strong	Regionally restricted	2J	Expression is restricted to the distal junctional complex of the ameloblasts in the maturation zone, with strong expression in the stratum intermedium.
TS28: stratum intermedium	Strong		2J

Structure	Level	Pattern	Image	Note
TS15: optic cup inner layer	Present	Widespread	3A	Expressed throughout the epithelium of the developing eye.
TS15: optic cup outer layer	Present		3A	Expression extended into the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS17: optic cup inner layer	Present		3B	Expressed in the epithelium of the optic cup.
TS17: lens pit	Present		3B	Expressed in the invaginating lens vesicle.

Structure	Level	Pattern	Image	Note
TS21: optic nerve	Strong		3C
TS21: pigmented retinal epithelium	Strong		3C

Structure	Level	Pattern	Image	Note
TS23: retina	Present	Regionally restricted	3D	Expressed in the area that will form the ciliary process.
TS23: optic nerve	Present		3D

Structure	Level	Pattern	Image	Note
TS27: ciliary body	Strong		3E

Structure	Level	Pattern	Image	Note
TS27: eye	Present	Regionally restricted	3F	Expressed in the epithelium around the optic nerve.

Structure	Level	Pattern	Image	Note
TS15: limb ectoderm	Present	Regionally restricted	3G	Expressed on the dorsal surface of the limb bud in a diffuse pattern throughout the apical region.
TS15: limb mesenchyme	Present	Regionally restricted	3G	Expressed on the dorsal surface of the limb bud in a diffuse pattern throughout the apical region.

Structure	Level	Pattern	Image	Note
TS20: limb epithelium	Present	Regionally restricted	3H	Expression was restricted to the epithelium of the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Strong	Regionally restricted	3I	Expressed in the midline epithelial seam of the fusing secondary palate.
TS22: nasal cavity epithelium	Strong	Regionally restricted	3I	Expressed in distinct regions.

Structure	Level	Pattern	Image	Note
TS23: vibrissa follicle	Strong		3J