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Gene Expression Data
Assay Details
Assay
Reference: J:206507 Kelsey L, et al., ENU-induced mutation in the DNA-binding domain of KLF3 reveals important roles for KLF3 in cardiovascular development and function in mice. PLoS Genet. 2013;9(7):e1003612
Assay type: RT-PCR
MGI Accession ID: MGI:5616882
Gene symbol: Hsd3b6
Gene name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 6
Probe: Hsd3b6-pA, Hsd3b6-pB
Probe preparation: labelled with SYBR green
Assay notes: This assay is a quantitative RT-PCR. 18S was used as the standard.
Results
Image: S10A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
WT E12.5 TS20: embryo Present 2.5 µg; total RNA involves: C3H/HeJ * C57BL/6J Not Specified
Het E12.5 TS20: embryo Present (a) 2.5 µg; total RNA involves: C3H/HeJ * C57BL/6J Klf3m1Cmhd/Klf3+Not Specified
Homo E12.5 TS20: embryo Present (b) 2.5 µg; total RNA involves: C3H/HeJ * C57BL/6J Klf3m1Cmhd/Klf3m1CmhdNot Specified
Notes:
(a) Expression was significantly diminished (0.45x) relative to wild-type littermate controls.
(b) Expression was significantly diminished (0.27x) relative to wild-type littermate controls.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory