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Gene Expression Data
Assay Details
Assay
Reference: J:135300 Babbs C, et al., Polydactyly in the mouse mutant Doublefoot involves altered Gli3 processing and is caused by a large deletion in cis to Indian hedgehog. Mech Dev. 2008 May-Jun;125(5-6):517-26
Assay type: Western blot
MGI Accession ID: MGI:5616767
Gene symbol: Gli3
Gene name: GLI-Kruppel family member GLI3
Antibody: anti-Gli3
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes: Forelimbs were dissected into anterior (A) and posterior (P) regions.
Results
Image: 5B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 190 kDa (a) 85 kDa (b) Genetic BackgroundMutant Allele(s)Sex Note
A E10.5 TS17: forelimb bud Present Strong (c) involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J Not Specified Anterior region of forelimb.
P E10.5 TS17: forelimb bud Present Strong involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J Not Specified Posterior region of forelimb.
A E10.5 TS17: forelimb bud Weak Weak involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J Dbf/Dbf+Not Specified Anterior region of forelimb.
P E10.5 TS17: forelimb bud Weak Weak involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J Dbf/Dbf+Not Specified Posterior region of forelimb.
A E10.5 TS17: forelimb bud Present Strong involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J Shhtm1Chg/Shhtm1ChgNot Specified Anterior region of forelimb.
P E10.5 TS17: forelimb bud Present Strong involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J Shhtm1Chg/Shhtm1ChgNot Specified Posterior region of forelimb.
A E10.5 TS17: forelimb bud Weak Weak involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J Dbf/Dbf+, Shhtm1Chg/Shhtm1ChgNot Specified Anterior region of forelimb.
P E10.5 TS17: forelimb bud Weak Weak involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J Dbf/Dbf+, Shhtm1Chg/Shhtm1ChgNot Specified Posterior region of forelimb.
Notes:
(a) This represents the Gli3A isoform.
(b) Band size was 83-86kDa. This represents the Gli3R isoform.
(c) Stronger in the anterior region.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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