Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:69788
Lee E, et al., Tissue-specific expression of the transcriptionally regulated serum and glucocorticoid-inducible protein kinase (Sgk) during mouse embryogenesis. Mech Dev. 2001 May;103(1-2):177-81
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:5616735
|
Gene symbol:
|
Sgk1
|
Gene name:
|
serum/glucocorticoid regulated kinase 1
|
Probe:
|
sgk probe1
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
1A:
embryonic day 8.5
(more )
Specimen
1A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: embryo |
Weak |
Diffuse |
1A
|
Generally diffuse pattern of low level expression throughout the embryo.
|
Specimen
1B:
embryonic day 9.5
(more )
Specimen
1B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: blood vessel |
Strong |
|
1B
|
Expression highly concentrated within lung buds and larger blood vessels.
|
TS15: lung |
Strong |
|
1B
|
Expression highly concentrated within lung buds and larger blood vessels.
|
Specimen
1C:
embryonic day 10.5
(more )
Specimen
1C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: heart |
Strong |
|
1C
|
Intensity of expression significantly increased in otic vesicle and heart chambers.
|
TS17: otocyst |
Strong |
|
1C
|
Intensity of expression significantly increased in otic vesicle and heart chambers.
|
Specimen
1D:
embryonic day 10.5
(more )
Specimen
1D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2B:
embryonic day 11.5
(more )
Specimen
2B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: heart |
Strong |
|
2B
|
|
TS19: blood vessel |
Strong |
Regionally restricted |
2B
|
Espression is high in blood vessels surrounding the somites.
|
Specimen
2C:
embryonic day 12.5
(more )
Specimen
2C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2D:
embryonic day 13.5
(more )
Specimen
2D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: heart |
Present |
|
2D
|
Significant expression maintained in the heart.
|
Specimen
2E:
embryonic day 14.5
(more )
Specimen
2E:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: heart |
Present |
|
2E
|
Significant expression maintained in the heart.
|
Specimen
not shown:
postnatal adult
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: heart |
Absent |
|
|
Authors report only background levels of expression in adult heart tissue.
|
Specimen
3A:
embryonic day 14.5
(more )
Specimen
3A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: heart |
Present |
|
3A
|
|
TS22: lung |
Strong |
Regionally restricted |
3A
|
High levels of expression in distal epithelium and terminal bronchioles.
|
Specimen
3B:
embryonic day 14.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: lung epithelium |
Strong |
Regionally restricted |
3B
|
High levels of expression in the distal epithelium.
|
Specimen
3D:
postnatal adult
(more )
Specimen
3D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: bronchus |
Strong |
|
3D
|
High levels of expression in the bronchi.
|
|