Gene Expression Data
Assay Details
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Assay
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Reference:
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J:73293
Pantaleon M, et al., FAM deubiquitylating enzyme is essential for preimplantation mouse embryo development. Mech Dev. 2001 Dec;109(2):151-60
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5616638
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Gene symbol:
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Faf1
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Gene name:
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Fas-associated factor 1
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Antibody:
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FAM antibody
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Detection system:
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Secondary antibody coupled to Texas Red
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Results
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Specimen
1C:
postnatal adult
(more  )
Note: Unfertilzed oocytes were flushed from uteri of superovulated unmated females.
Specimen
1C:
(close  )
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Genetic Background:
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Quackenbush
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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Unfertilzed oocytes were flushed from uteri of superovulated unmated females.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: secondary oocyte |
Strong |
Regionally restricted |
1C
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Strong localization to spindle and chromosomes; lower levels in the cytoplasm.
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Specimen
1D:
embryonic day 0.5
(more )
Note: Fertilzed eggs were collected from mated females.
Specimen
1D:
(close )
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Genetic Background:
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Quackenbush
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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Fertilzed eggs were collected from mated females.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Strong |
Regionally restricted |
1D
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Expression is significantly upregulated in the cytoplasm. No expression in male or female pronuclei.
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Specimen
1E:
embryonic day 1.0
(more )
Specimen
1E:
(close )
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Genetic Background:
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Quackenbush
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Strong |
Regionally restricted |
1E
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Expression mainly associated with perinuclear membrane; also apparent in cytoplasm. No expression in chromosomes or spindle.
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Specimen
1F:
embryonic day 2.0
(more )
Specimen
1F:
(close )
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Genetic Background:
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Quackenbush
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Age:
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embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 4-cell stage embryo |
Strong |
Regionally restricted |
1F
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Expression mainly associated with perinuclear membrane; also apparent in cytoplasm. No expression in chromosomes or spindle.
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Specimen
1G:
embryonic day 3.0
(more )
Specimen
1G:
(close )
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Genetic Background:
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Quackenbush
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: compacted morula |
Strong |
Regionally restricted |
1G
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Expression pattern maintained following initiation of differentiation at compaction.
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Specimen
1H:
embryonic day 4.0
(more )
Specimen
1H:
(close )
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Genetic Background:
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Quackenbush
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Age:
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embryonic day 4.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Strong |
Regionally restricted |
1H
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Expression pattern maintained in the blastocyst.
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| TS4: inner cell mass |
Present |
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1H
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No discernible difference of expression between cells of the inner cell mass and the trophectoderm.
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| TS4: trophectoderm |
Present |
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1H
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No discernible difference of expression between cells of the inner cell mass and the trophectoderm.
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Analysis Tools