Gene Expression Data
Assay Details
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Assay
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Reference:
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J:135300
Babbs C, et al., Polydactyly in the mouse mutant Doublefoot involves altered Gli3 processing and is caused by a large deletion in cis to Indian hedgehog. Mech Dev. 2008 May-Jun;125(5-6):517-26
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5616593
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh probe102
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
3A:
embryonic day 10.5
(more  )
Specimen
3A:
(close  )
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Genetic Background:
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involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
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3A
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Expression is at the posterior margin.
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Specimen
3B:
embryonic day 10.5; Dbf/Dbf +
(more )
Specimen
3B:
(close )
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Genetic Background:
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involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J
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Mutant Allele(s):
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Dbf/Dbf+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
3B
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Expression in this mutant is the same as in wild-type embryos.
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Specimen
3D:
embryonic day 10.5; Dbf/Dbf +
(more )
Note: Double labeled: blue - Shh; blue - Ihh (assay MGI:5616601).
Specimen
3D:
(close )
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Genetic Background:
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involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J
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Mutant Allele(s):
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Dbf/Dbf+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: blue - Shh; blue - Ihh (assay MGI:5616601).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
3D
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Expression is at the posterior margin and is the same as in wild-type embryos.
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Specimen
3F:
embryonic day 11.5
(more )
Specimen
3F:
(close )
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Genetic Background:
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involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
3F
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Expression is at the posterior margin.
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Specimen
3G:
embryonic day 11.5; Dbf/Dbf +
(more )
Specimen
3G:
(close )
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Genetic Background:
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involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J
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Mutant Allele(s):
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Dbf/Dbf+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
3G
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Expression in this mutant is the same as in wild-type embryos.
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Analysis Tools