Gene Expression Data
Assay Details
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Assay
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Reference:
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J:135300
Babbs C, et al., Polydactyly in the mouse mutant Doublefoot involves altered Gli3 processing and is caused by a large deletion in cis to Indian hedgehog. Mech Dev. 2008 May-Jun;125(5-6):517-26
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5616591
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Gene symbol:
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Hand2
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Gene name:
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heart and neural crest derivatives expressed 2
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Probe:
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Hand2 probe5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1C:
embryonic day 9.5
(more  )
Specimen
1C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud |
Present |
Regionally restricted |
1C
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Expression is in the posterior region.
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Specimen
1D:
embryonic day 9.5; Dbf/Dbf +
(more )
Specimen
1D:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH
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Mutant Allele(s):
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Dbf/Dbf+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud |
Present |
Regionally restricted |
1D
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Expression in this mutant is the same as in wild-type embryos.
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Specimen
1G:
embryonic day 11.5
(more )
Specimen
1G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
1G
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Expression is at the proximal posterior margin.
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Specimen
1H:
embryonic day 11.5; Dbf/Dbf +
(more )
Specimen
1H:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH
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Mutant Allele(s):
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Dbf/Dbf+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
1H
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Expression extends anteriorly and distally in this mutant.
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Specimen
4 wt:
embryonic day 10.5
(more )
Specimen
4 wt:
(close )
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Genetic Background:
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involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4 Dbf/+:
embryonic day 10.5; Dbf/Dbf +
(more )
Specimen
4 Dbf/+:
(close )
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Genetic Background:
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involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J
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Mutant Allele(s):
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Dbf/Dbf+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4 Shh-/-:
(close )
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Genetic Background:
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involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4 Shh-/-;Dbf/+:
(close )
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Genetic Background:
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involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J
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Mutant Allele(s):
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Dbf/Dbf+
Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Analysis Tools