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Gene Expression Data
Assay Details
Assay
Reference: J:202263 Jolly LA, et al., The UPF3B gene, implicated in intellectual disability, autism, ADHD and childhood onset schizophrenia regulates neural progenitor cell behaviour and neuronal outgrowth. Hum Mol Genet. 2013 Dec 1;22(23):4673-87
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5615970
Gene symbol: Upf3b
Gene name: UPF3 regulator of nonsense transcripts homolog B (yeast)
Antibody: Anti-Upf3b
Detection system: Secondary antibody
Assay notes: Two antibodies were described MGI:5615934 and MGI:5615965. Either may have been used in this assay.
Results
Specimen 1A ii/iii: embryonic day 14.5 (more )
Note: iii also labeled with Dapi.
Structure Level Pattern Image Note
TS22: cortical plate Present 1A Upf3b ii , 1A Upf3b iii
Specimen 1A iii Stau1/Upf3b: embryonic day 14.5 (more )
Note: Double labeled: red - Upf3b; green - Stau1 (assay MGI:5615976).
Structure Level Pattern Image Note
TS22: cortical plate Present 1A Stau1/Upf3b iii
Specimen 1A iii Upf3a/Upf3b: embryonic day 14.5 (more )
Note: Double labeled: red - Upf3b; green - Upf3a (assay MGI:5615974).
Structure Level Pattern Image Note
TS22: cortical plate Present 1A Upf3a/Upf3b iii
Specimen 1A iii Upf1/Upf3b: embryonic day 14.5 (more )
Note: Double labeled: red - Upf3b; green - Upf1 (assay MGI:5615977).
Structure Level Pattern Image Note
TS22: cortical plate Present 1A Upf1/Upf3b iii
Specimen 1B E10.5: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: telencephalon ventricular layer Present 1B E10.5
Specimen 1B E14.5: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: cerebral cortex ventricular layer Present 1B E14.5 VZ , 1B E14.5 left
TS22: cortical plate Present 1B E14.5 left , 1B E14.5 CP
Specimen 1B E18.5: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: cerebral cortex ventricular zone Present 1B E18.5 left , 1B E18.5 VZ
TS26: cortical plate Present 1B E18.5 CP , 1B E18.5 left
Specimen 2C Hippocampus: postnatal adult (more )
Note: Lower image also labeled with Dapi.
Structure Level Pattern Image Note
TS28: hippocampus Present 2C Hippocampus upper Upf3b , 2C Hippocampus lower Upf3b
Specimen 2C Hippocampus Upf3a/Upf3b: postnatal adult (more )
Note: Double labeled: red - Upf3b; green - Upf3a (assay MGI:5615974).
Structure Level Pattern Image Note
TS28: hippocampus Present 2C Hippocampus Upf3a/Upf3b
Specimen 2C Hippocampus Stau1/Upf3b: postnatal adult (more )
Note: Double labeled: red - Upf3b; green - Stau1 (assay MGI:5615976).
Structure Level Pattern Image Note
TS28: hippocampus Present 2C Hippocampus Stau1/Upf3b
Specimen 2C Hippocampus Upf3b/Stau1/Upf3a: postnatal adult (more )
Note: Triple labeled: red - Upf3b; green - Stau1 (assay MGI:5615976); blue - Upf3a (assay MGI:5615974).
Structure Level Pattern Image Note
TS28: hippocampus Present 2C Hippocampus Upf3b/Stau1/Upf3a
Specimen 2C Hippocampal CA2: postnatal adult (more )
Note: Lower image also labeled with Dapi.
Structure Level Pattern Image Note
TS28: hippocampus CA2 Present 2C Hippocampal CA2 lower Upf3b , 2C Hippocampal CA2 upper Upf3b
Specimen 2C Hippocampal CA2 Upf3a/Upf3b: postnatal adult (more )
Note: Double labeled: red - Upf3b; green - Upf3a (assay MGI:5615974).
Structure Level Pattern Image Note
TS28: hippocampus CA2 Present 2C Hippocampal CA2 Upf3a/Upf3b
Specimen 2C Hippocampal CA2 Stau1/Upf3b: postnatal adult (more )
Note: Double labeled: red - Upf3b; green - Stau1 (assay MGI:5615976).
Structure Level Pattern Image Note
TS28: hippocampus CA2 Present 2C Hippocampal CA2 Stau1/Upf3b
Specimen 2C Hippocampal CA2 Upf3b/Stau1/Upf3a: postnatal adult (more )
Note: Triple labeled: red - Upf3b; green - Stau1 (assay MGI:5615976); blue - Upf3a (assay MGI:5615974).
Structure Level Pattern Image Note
TS28: hippocampus CA2 Present 2C Hippocampal CA2 Upf3b/Stau1/Upf3a
Specimen S6: embryonic day 18.5 (more )
Note: Triple labeled: red - Upf3b; green - Stau1 (assay MGI:5615976); blue - Upf3a (assay MGI:5615974).
Structure Level Pattern Image Note
TS26: cortical plate Present S6_left , S6_CP/SP , S6_MZ/CP
TS26: cerebral cortex ventricular zone Present S6_left , S6_SP/SVZ/VZ
Specimen S7: embryonic day 14.5 (more )
Note: Left - single label. Right - double labeled: red - Upf3b; green - Tubb3 (assay MGI:5616149).
Structure Level Pattern Image Note
TS22: cerebral cortex ventricular layer Present S7_left , S7_right Expression was in neuronal progenitor cells.
TS22: cortical plate Present S7_right , S7_left Expression was in neurons.
Specimen S9A: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: cerebral cortex ventricular layer Present S9A Upf3b Staining
TS22: cortical plate Present S9A Upf3b Staining
Specimen S10: postnatal adult (more )
Note: Lower image also labeled with Dapi.
Structure Level Pattern Image Note
TS28: cerebral cortex Present S10_Upf3b lower , S10_Upf3b upper
Specimen S10 Upf3a/Upf3b: postnatal adult (more )
Note: Double labeled: red - Upf3b; green - Upf3a (assay MGI:5615974).
Structure Level Pattern Image Note
TS28: cerebral cortex Present S10_Upf3a/Upf3b
Specimen S10 Stau1/Upf3b: postnatal adult (more )
Note: Double labeled: red - Upf3b; green - Stau1 (assay MGI:5615976).
Structure Level Pattern Image Note
TS28: cerebral cortex Present S10_Stau1/Upf3b
Specimen S10 Upf3b/Stau1/Upf3a: postnatal adult (more )
Note: Triple labeled: red - Upf3b; green - Stau1 (assay MGI:5615976); blue - Upf3a (assay MGI:5615974).
Structure Level Pattern Image Note
TS28: cerebral cortex Present S10_Upf3b/Stau1/Upf3a
Specimen S11A Cerebellum: postnatal adult (more )
Note: Lower image also labeled with Dapi.
Structure Level Pattern Image Note
TS28: cerebellum Present S11A Cerebellum Upf3b lower , S11A Cerebellum Upf3b upper
Specimen S11A Cerebellum Upf3a/Upf3b: postnatal adult (more )
Note: Double labeled: red - Upf3b; green - Upf3a (assay MGI:5615974).
Structure Level Pattern Image Note
TS28: cerebellum Present S11A Cerebellum Upf3a/Upf3b
Specimen S11A Cerebellum Stau1/Upf3b: postnatal adult (more )
Note: Double labeled: red - Upf3b; green - Stau1 (assay MGI:5615976).
Structure Level Pattern Image Note
TS28: cerebellum Present S11A Cerebellum Stau1/Upf3b
Specimen S11A Cerebellum Upf3b/Stau1/Upf3a: postnatal adult (more )
Note: Triple labeled: red - Upf3b; green - Stau1 (assay MGI:5615976); blue - Upf3a (assay MGI:5615974).
Structure Level Pattern Image Note
TS28: cerebellum Present S11A Upf3b/Stau1/Upf3a Cerebellum
Specimen S11A Purkinje cells: postnatal adult (more )
Note: Lower image also labeled with Dapi.
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Present S11A Purkinje cells Upf3b lower , S11A Purkinje cells Upf3b upper
Specimen S11A Purkinje cells Upf3a/Upf3b: postnatal adult (more )
Note: Double labeled: red - Upf3b; green - Upf3a (assay MGI:5615974).
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Present S11A Purkinje cells Upf3a/Upf3b
Specimen S11A Purkinje cells Stau1/Upf3b: postnatal adult (more )
Note: Double labeled: red - Upf3b; green - Stau1 (assay MGI:5615976).
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Present S11A Purkinje cells Stau1/Upf3b
Specimen S11A Purkinje cells Upf3b/Stau1/Upf3a: postnatal adult (more )
Note: Triple labeled: red - Upf3b; green - Stau1 (assay MGI:5615976); blue - Upf3a (assay MGI:5615974).
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Present S11A Upf3b/Stau1/Upf3a Purkinje cells
Specimen S11B left: postnatal adult (more )
Note: Double labeled: red - Upf3b; green - Upf3a (assay MGI:5615974).
Structure Level Pattern Image Note
TS28: cerebellum Present S11B left
Specimen S11B right: postnatal adult (more )
Note: Double labeled: red - Upf3b; green - Gfap (assay MGI:5616180).
Structure Level Pattern Image Note
TS28: cerebellum Present S11B right
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory