About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:217513 Espinoza-Lewis RA, et al., Generation of a Cre knock-in into the Myocardin locus to mark early cardiac and smooth muscle cell lineages. Genesis. 2014 Oct;52(10):879-87
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5615368
Gene symbol: Isl1
Gene name: ISL1 transcription factor, LIM/homeodomain
Antibody: clone 39.4D5
Detection system: Secondary antibody coupled to horseradish peroxidase
Results
Specimen 5A E7.75 WT: embryonic day 7.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Structure Level Pattern Image Note
TS11: heart Present 5A E7.75 WT
Specimen 5A E7.75 KI/+: embryonic day 7.75; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant. Double labeled: red - Isl1; blue - Cre reporter
Structure Level Pattern Image Note
TS11: heart Present 5A E7.75 KI
Specimen 5A E8.0 WT: embryonic day 8.0; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Structure Level Pattern Image Note
TS12: primitive heart tube Present Regionally restricted 5A E8.0 WT staining at the poles of the forming tubular heart
Specimen 5A E8.0 KI/+: embryonic day 8.0; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant. Double labeled: red - Isl1; blue - Cre reporter
Structure Level Pattern Image Note
TS12: primitive heart tube Present Regionally restricted 5A E8.0 KI staining at the poles of the forming tubular heart
Specimen 5B E7.75 WT: embryonic day 7.75; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Structure Level Pattern Image Note
TS11: heart Present 5B E7.75 WT
Specimen 5B E7.75 KI/+: embryonic day 7.75; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant. Transversal sections. Double labeled: red - Isl1; blue - Cre reporter
Structure Level Pattern Image Note
TS11: heart Present 5B E7.75 KI
Specimen 5B E8.0 WT: embryonic day 8.0; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Structure Level Pattern Image Note
TS12: primitive heart tube Present 5B E8.0 WT
Specimen 5B E8.0 KI/+: embryonic day 8.0; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant. Transversal sections. Double labeled: red - Isl1; blue - Cre reporter
Structure Level Pattern Image Note
TS12: primitive heart tube Present 5B E8.0 KI
Specimen 5C left: embryonic day 7.75; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant. Transversal sections. Double labeled: red - Isl1; blue - Cre reporter
Structure Level Pattern Image Note
TS11: heart Present 5C left
Specimen 5C right: embryonic day 7.75; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Myocdtm1(cre)Dzw/Myocd+ (more )
Note: Conditional mutant. Transversal sections. Double labeled: red - Isl1; blue - Cre reporter
Structure Level Pattern Image Note
TS11: heart Present 5C right
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
12/30/2025
MGI 6.24 		The Jackson Laboratory