Gene Expression Data
Assay Details
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Assay
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Reference:
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J:3904
Deltour L, et al., Differential expression of the two nonallelic proinsulin genes in the developing mouse embryo. Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):527-31
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:5614603
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Gene symbol:
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Ins2
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Gene name:
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insulin II
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Probe:
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Ins2-pI, Ins1/2-pD
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Probe preparation:
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Double stranded labelled with P32 DNA
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Visualized with:
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Autoradiography
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Assay notes:
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The primer set used for this assay amplifies both insulin genes. Endonuclease digestion (with MspI) was then used to determine which gene products had been amplified. The blot was hybridized to a probe homologous to both mRNAs (MGI:5614574).
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Results
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| E9.5
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TS15: head
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Present
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2.5 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
| E10.5
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TS17: head
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Present
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2.5 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
| E10.5
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TS17: head
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Present
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2.5 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
| E11.5
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TS19: head
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Present
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2.5 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
| E11.5
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TS19: head
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Present
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2.5 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
| E12.5
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TS20: head
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Present
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2.5 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
| E12.5
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TS20: head
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Present
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2.5 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
| E13.5
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TS21: head
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Present
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2.5 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
| E14.5
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TS22: head
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Present
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2.5 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
| E14.5
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TS22: head
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Present
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2.5 µg; total RNA |
(C57BL/6 x DBA/2)F1 |
| Not Specified |
| Control |
| Mol. Wt. Marker Lane |
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Analysis Tools