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Gene Expression Data
Assay Details
Assay
Reference: J:3904 Deltour L, et al., Differential expression of the two nonallelic proinsulin genes in the developing mouse embryo. Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):527-31
Assay type: RT-PCR
MGI Accession ID: MGI:5614601
Gene symbol: Ins2
Gene name: insulin II
Probe: Ins2-pI, Ins1/2-pD
Probe preparation: Double stranded labelled with P32 DNA
Visualized with: Autoradiography
Assay notes: The primer set used for this assay amplifies both insulin genes. Endonuclease digestion (with MspI) was then used to determine which gene products had been amplified. The blot was hybridized to a probe homologous to both mRNAs (MGI:5614574).
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
8.5 E8.5 TS13: embryo Present (a) 2.5 µg; total RNA (C57BL/6 x DBA/2)F1 Not Specified
9.5 E9.5 TS15: trunk Present (b) 2.5 µg; total RNA (C57BL/6 x DBA/2)F1 Not Specified decapitated embryos were used
10.5 E10.5 TS17: trunk Present (b) 2.5 µg; total RNA (C57BL/6 x DBA/2)F1 Not Specified decapitated embryos were used
10.5 E10.5 TS17: trunk Present (b) 2.5 µg; total RNA (C57BL/6 x DBA/2)F1 Not Specified decapitated embryos were used
11.5 E11.5 TS19: trunk Present 2.5 µg; total RNA (C57BL/6 x DBA/2)F1 Not Specified decapitated embryos were used
11.5 E11.5 TS19: trunk Present 2.5 µg; total RNA (C57BL/6 x DBA/2)F1 Not Specified decapitated embryos were used
12.5 E12.5 TS20: trunk Present (c) 2.5 µg; total RNA (C57BL/6 x DBA/2)F1 Not Specified decapitated embryos were used
12.5 E12.5 TS20: trunk Present (c) 2.5 µg; total RNA (C57BL/6 x DBA/2)F1 Not Specified decapitated embryos were used
13.5 E13.5 TS21: trunk Present (c) 2.5 µg; total RNA (C57BL/6 x DBA/2)F1 Not Specified decapitated embryos were used
13.5 E13.5 TS21: trunk Present (c) 2.5 µg; total RNA (C57BL/6 x DBA/2)F1 Not Specified decapitated embryos were used
14.5 E14.5 TS22: trunk Present (c) 2.5 µg; total RNA (C57BL/6 x DBA/2)F1 Not Specified decapitated embryos were used
M Mol. Wt. Marker Lane
Notes:
(a) Expression of proinsulin II is observed before the appearance of the pancreatic anlage.
(b) There are fewer proinsulin II mRNAs than proinsulin I mRNAs at this time point.
(c) There are more proinsulin II mRNAs than proinsulin I mRNAs at this time point.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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