Antibody:	Anti-Lpp3
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	4C	Expression was detected in the ventral midbrain.
TS20: hindbrain	Present		4C
TS20: spinal cord	Present	Regionally restricted	4C	Expression was detected in spinal dorsal interneurons.
TS20: neurohypophysis infundibulum	Weak		4C
TS20: thalamus	Weak	Regionally restricted	4C	Expression was weak in the thalamus ventricular area.
TS20: dorsal root ganglion	Present	Graded	4C	Expression was stronger in posterior than in anterior ganglia.
TS20: trigeminal ganglion	Present	Non-Uniform	4C , 4F	Expression was weak in the body of the trigeminal ganglion, but strong in projecting axons.

Structure	Level	Pattern	Image	Note
TS20: skin	Present	Regionally restricted	4E	Expression was detected in the mammary gland primordia.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	4G	Expression was detected in the spinal dorsal interneurons, the ependymal layer, and a subset of motor neurons.
TS20: dorsal root ganglion	Present		4G

Structure	Level	Pattern	Image	Note
TS18: cranial nerve	Very strong		4H
TS18: spinal nerve	Very strong		4H
TS18: neural tube ventricular layer	Weak		4H

Structure	Level	Pattern	Image	Note
TS18: spinal nerve	Very strong		4H inset
TS18: neural tube ventricular layer	Weak		4H inset

Structure	Level	Pattern	Image	Note
TS18: forelimb bud apical ectodermal ridge	Strong		4I
TS18: forelimb bud mesenchyme	Moderate	Regionally restricted	4I	Expression was detected in the progress zone mesenchyme and the proximal dorsomedial mesenchyme of the forelimb. Expression in the progress zone area was more restricted to the dorsal side.