Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS13: allantois	Present		2B
TS13: future hindbrain	Present		2B
TS13: future midbrain	Present		2B
TS13: paraxial mesenchyme	Present		2B
TS13: future prosencephalon	Absent		2B
TS13: embryo	Weak	Regionally restricted	2B	Expression was weak in the posterior embryonic domain.

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present		2C
TS13: pericardio-peritoneal canal mesothelium	Present		2C
TS13: somite	Present		2C
TS13: future spinal cord	Present	Regionally restricted	2C	Expression was detected in the ventral portion of the future spinal cord.

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present		2D
TS13: future spinal cord	Present	Regionally restricted	2D	Expression was detected in the ventral portion of the future spinal cord.

Structure	Level	Pattern	Image	Note
TS13: future spinal cord	Present	Regionally restricted	2E	Expression was detected in the ventral portion of the future spinal cord.
TS13: notochord	Present		2E

Structure	Level	Pattern	Image	Note
TS13: amnion	Present
TS13: head	Present
TS13: embryo mesenchyme	Present	Regionally restricted		Expression was detected in the cervical mesoderm.
TS13: yolk sac mesenchyme	Weak	Regionally restricted		Expression was low in the vascular mesodermal cells of the yolk sac.

Structure	Level	Pattern	Image	Note
TS14: ear	Strong	Regionally restricted	2G	Strongest expression was detected in the endoderm and ectoderm epithelia of the future tympanic membrane region.
TS14: allantois	Present		2G
TS14: intraembryonic coelom	Present		2G
TS14: somite	Present		2G
TS14: paraxial mesenchyme	Present	Regionally restricted	2G	Expression was detected in the paraxial tissue at the cervical region.

Structure	Level	Pattern	Image	Note
TS14: head mesenchyme	Present	Regionally restricted	2H	Expression was detected in the cephalic mesenchyme except in the telencephalic area.
TS14: embryo mesenchyme	Present	Regionally restricted	2H	Expression was detected in the cervical mesenchyme.
TS14: nervous system	Present		2H
TS14: notochord	Present		2H

Structure	Level	Pattern	Image	Note
TS14: outflow tract	Present		2I
TS14: common atrial chamber	Present	Regionally restricted	2I	Expression was detected in the posterior wall of the common atrial chamber.
TS14: heart	Present	Regionally restricted	2I	Expression was in a few cells of the endocardial lining in the bulbo-ventricular canal.
TS14: thyroid primordium	Present		2I
TS14: foregut	Present	Regionally restricted	2I	Expression was detected in the ventral aspect of the foregut endoderm.

Structure	Level	Pattern	Image	Note
TS14: foregut-midgut junction	Weak		2J
TS14: hepatic primordium	Weak		2J
TS14: hindgut diverticulum	Weak		2J
TS14: pericardio-peritoneal canal mesothelium	Strong		2J

Structure	Level	Pattern	Image	Note
TS14: inner ear epithelium	Present	Regionally restricted		Expression was detected in the otic vesicle epithelium.
TS14: migrating neural crest	Present	Regionally restricted		Expression was detected in migratory neural crest tissue.

Structure	Level	Pattern	Image	Note
TS18: embryo mesenchyme	Present	Non-Uniform	2L	Expression was detected in the cephalic and cervical regions. Expression in the cephalic flexure mesenchyme and surrounding the primary head and anterior cardinal vein and dorsal aorta was more intense.
TS18: head mesenchyme	Present	Non-Uniform	2L
TS18: 1st branchial arch maxillary component mesenchyme	Present	Regionally restricted	2L	Expression was detected in the anterior distal aspect.
TS18: 2nd branchial arch mesenchyme	Present		2L
TS18: dermomyotome	Present	Non-Uniform	2L	Expression in the dermomyotome of epithelialized somites was present in the caudal region whereas at the cervical and thoracic levels, expression was gradually restricted to the dorsal lip region.

Structure	Level	Pattern	Image	Note
TS18: midbrain floor plate	Present		2M

Structure	Level	Pattern	Image	Note
TS18: brain floor plate	Present		2N

Structure	Level	Pattern	Image	Note
TS18: hindbrain basal plate	Present		2O
TS18: hindbrain floor plate	Present		2O
TS18: trigeminal ganglion	Present		2O

Structure	Level	Pattern	Image	Note
TS18: acoustic ganglion	Present		2P
TS18: facial ganglion	Present		2P
TS18: hindbrain basal plate	Present		2P
TS18: hindbrain floor plate	Present		2P
TS18: head mesenchyme derived from neural crest	Strong	Regionally restricted	2P	Stronger expression was detected in the interacting region between the otic vesicle epithelia and the neural crest cells of the acoustic preganglion.

Structure	Level	Pattern	Image	Note
TS18: heart	Present	Regionally restricted	2Q	Expression corresponded to the most dorsal aspect of the heart and was in cells of the dorsal truncus arteriosus.
TS18: body wall	Present	Regionally restricted	2Q	Expression was detected in the body wall overlaying the pericardial cavity.
TS18: endocardial cushion	Present		2Q
TS18: blood vessel	Present		2Q	Expression was detected in blood vessel endothelial cells.

Structure	Level	Pattern	Image	Note
TS18: bronchus mesenchyme	Moderate
TS18: dorsal mesocardium	Moderate
TS18: esophagus	Moderate
TS18: forebrain	Present	Regionally restricted		Expression was not detected in the neuroepithelium. Detectable expression correlated with invading blood vessels from the perineural vascular plexus.
TS18: forebrain vascular element	Present	Regionally restricted

Structure	Level	Pattern	Image	Note
TS18: future spinal cord	Present	Regionally restricted	2R	Expression was detected in a group of presumptive motor neurons at the thoracic and lumbar areas.

Structure	Level	Pattern	Image	Note
TS17: lens pit	Present	Regionally restricted		Expression was found in the central domain.
TS17: optic cup outer layer	Present	Regionally restricted		Expression was found in the central domain.

Structure	Level	Pattern	Image	Note
TS18: paraxial mesenchyme	Present	Non-Uniform	2S	Expression was more abundant in the anterior part of the paraxial mesenchyme.
TS18: notochord	Present		2S
TS18: dorsal root ganglion	Weak		2S

Structure	Level	Pattern	Image	Note
TS18: paraxial mesenchyme	Present	Non-Uniform	2T	Expression was less abundant in the posterior part of the paraxial mesenchyme.
TS18: genitourinary system	Present	Regionally restricted	2T	Expression was detected in a bilateral stripe of mesothelial cells contiguous to the dorsal mesentery and the mesenchyme adjacent to the dorsal aorta.
TS18: dorsal root ganglion	Weak		2T

Structure	Level	Pattern	Image	Note
TS15: forelimb bud ectoderm	Strong	Scattered	3A	Expression was detected in a group of scattered ectodermal cells in the ventral side of the developing forelimb.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud apical ectodermal ridge	Strong		3B
TS18: forelimb bud mesenchyme	Moderate	Regionally restricted	3B	Expression was detected in the progress zone mesenchyme and the proximal dorsomedial mesenchyme of the forelimb. Expression in the progress zone area was more restricted to the dorsal side.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud apical ectodermal ridge	Strong		3C
TS18: forelimb bud mesenchyme	Moderate	Regionally restricted	3C	Expression was detected in the progress zone mesenchyme and the proximal dorsomedial mesenchyme of the forelimb. Expression in the progress zone area was more restricted to the dorsal side.

Structure	Level	Pattern	Image	Note
TS19: handplate apical ectodermal ridge	Present		3D
TS19: future arm mesenchyme	Weak	Regionally restricted	3D	Weaker staining was observed in the superficial dorsomedial mesenchyme of the zeugopod.
TS19: handplate	Strong	Regionally restricted	3D	Strong expression was detected in the dorsal-central domain of the autopod. Weak expression was detected in the future digit domains.
TS19: epithelium	Present	Regionally restricted	3D	Expression was detected in the developing mammary gland placodes 3 and 4.

Structure	Level	Pattern	Image	Note
TS19: paraxial mesenchyme	Present	Regionally restricted		At the hindlimb level, expression was abundant in the paraxial mesenchyme ventral to the inter dorsal root ganglia space.
TS19: physiological umbilical hernia	Strong
TS19: urogenital sinus	Strong

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present	Regionally restricted	3E	Expression was detected in the mesenchyme surrounding the marginal vein. Expression in the dorsal muscle anlagen was evident.
TS20: hand	Present	Regionally restricted	3E	Expression was stronger in the distal region of the prospective digits. The dorsal central domain of expression in the autopod was wider.

Structure	Level	Pattern	Image	Note
TS21: forelimb digit	Present	Regionally restricted	3F left	Expression was detected in the most central cells of the digits and the tip of the digits.
TS21: perichondrium	Present		3F left
TS21: connective tissue	Present	Regionally restricted	3F left	Expression was detected in developing ventral tendons.

Structure	Level	Pattern	Image	Note
TS21: forelimb digit	Present	Regionally restricted	3F middle	Expression was detected in the most central cells of the digits and the tip of the digits.
TS21: perichondrium	Present		3F middle

Structure	Level	Pattern	Image	Note
TS21: hindlimb digit	Present	Regionally restricted	3F right	Expression was detected in the most central cells of the digits and the tip of the digits.
TS21: perichondrium	Present		3F right

Structure	Level	Pattern	Image	Note
TS21: mammary gland primordium epithelium	Strong		3G , 3H
TS21: mammary mesenchyme	Weak		3G , 3H
TS21: forelimb digit	Present	Regionally restricted	3G	Expression was detected in the most central cells of the digits and the tip of the digits.
TS21: perichondrium	Present		3G

Structure	Level	Pattern	Image	Note
TS21: skeletal muscle	Present	Regionally restricted		Expression was evident in the dorsal and ventral muscle anlagen.

Structure	Level	Pattern	Image	Note
TS20: dermis	Present		4B
TS20: skin	Present	Regionally restricted	4B	Expression was abundant in the five pairs of mammary gland placodes.
TS20: head mesenchyme	Present	Regionally restricted	4B	Abundant expression was more evident around the primary head vein and the cephalic ganglia.
TS20: myotome	Present	Regionally restricted	4B	Expression was detected in the most anterior myotomes at the inter limb area and more restricted to their dorsal lip in the rest of the embryo.
TS20: tail somite	Present	Regionally restricted	4B	Expression was detected in tail somites but absent in its distal extremity.
TS20: forelimb mesenchyme	Present	Regionally restricted	4B	Expression was detected in the mesenchyme surrounding the marginal vein. Expression in the dorsal muscle anlagen was evident.
TS20: hand	Present	Regionally restricted	4B	Expression was stronger in the distal region of the prospective digits. The dorsal central domain of expression in the autopod was wider.

Structure	Level	Pattern	Image	Note
TS20: liver	Absent
TS20: endocardial cushion	Present	Regionally restricted		Expression was detected in the atrioventricular endocardial cushions.
TS20: nephric duct, mesonephric portion	Present	Regionally restricted		Expression was detected in the epithelium.
TS20: urethral plate	Present	Regionally restricted		Expression was detected in the epithelium.
TS20: genital tubercle mesenchyme	Present			Expression was in deep mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present		5A
TS21: cartilage condensation	Ambiguous		5A	Expression was very low or undetectable.
TS21: vibrissa follicle	Ambiguous		5A	Expression was very low or undetectable.
TS21: mammary gland primordium epithelium	Strong		5A
TS21: mammary mesenchyme	Weak		5A

Structure	Level	Pattern	Image	Note
TS21: cardiac muscle tissue	Ambiguous		5B
TS21: liver	Ambiguous		5B
TS21: cartilage condensation	Ambiguous		5B	Expression in condensed cartilage was very low or undetectable.

Structure	Level	Pattern	Image	Note
TS21: atrioventricular valve	Present		5C
TS21: cerebellum ventricular layer	Present		5C
TS21: cerebral cortex	Present		5C
TS21: heart atrium	Present		5C
TS21: hindbrain ventricular layer	Present		5C
TS21: midbrain ventricular layer	Present		5C
TS21: vena cava	Present		5C
TS21: cartilage	Present	Non-Uniform	5C	Expression varied in some regions of highly differentiated cartilage.
TS21: dorsal root ganglion	Weak		5C
TS21: perichondrium	Present	Non-Uniform	5C	Expression was strong to moderate.

Structure	Level	Pattern	Image	Note
TS21: atrioventricular valve	Present		5E , 5D
TS21: heart atrium	Present		5D

Structure	Level	Pattern	Image	Note
TS21: vena cava	Present		5F
TS21: vertebral cartilage condensation	Present		5F
TS21: dorsal root ganglion	Weak		5F

Structure	Level	Pattern	Image	Note
TS21: vertebral cartilage condensation	Present		5G

Structure	Level	Pattern	Image	Note
TS21: spinal cord ventricular layer	Present	Regionally restricted	5H	Expression was detected in a stripe running along the central domain of the ependymal layer with weaker staining extended to the whole ependymal layer.
TS21: dorsal root ganglion	Weak		5H

Structure	Level	Pattern	Image	Note
TS21: spinal cord ventricular layer	Present	Regionally restricted	5I	Expression was detected in a stripe running along the central domain of the ependymal layer with weaker staining extended to the whole ependymal layer.

Structure	Level	Pattern	Image	Note
TS21: spinal cord ventricular layer	Present	Regionally restricted	5I inset	Expression was detected in the central domain of the ependymal layer with weaker staining extended to the whole ependymal layer.

Structure	Level	Pattern	Image	Note
TS23: mammary gland primordium epithelium	Present			Expression gradually diminishes.
TS23: mammary mesenchyme	Present			Progressively expressed in the mesenchyme surrounding epithelial placodes and mammary buds.

Structure	Level	Pattern	Image	Note
TS24: mammary mesenchyme	Present			Progressively expressed in the mesenchyme surrounding epithelial placodes and mammary buds.
TS24: mammary gland primordium epithelium	Ambiguous			Expression gradually diminishes from E15.5-16.5.