Antibody:	anti c-kit
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: neural tube	Weak	Uniform	5A

Structure	Level	Pattern	Image	Note
TS17: neural tube	Strong	Regionally restricted	5B	Expression was strong in the dorsal half of the neural tube. The abnormal dorsal expression was throughout the length of the neural tube except in the anterior trunk regions, where the roof plate was devoid of expression.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	5C	The expression pattern in the homozygous mutant was similar to the heterozygous embryo.

Structure	Level	Pattern	Image	Note
TS17: dermatome	Trace		5D

Structure	Level	Pattern	Image	Note
TS17: dermatome	Present		5E	Expression was abnormally high in the heterozygous embryo compared to wild type.

Structure	Level	Pattern	Image	Note
TS17: hindgut mesentery	Strong
TS17: primordial germ cell	Strong
TS17: cardiovascular system mesenchyme	Strong	Regionally restricted		Strong expression was detected in the mesenchyme around the dorsal aorta.
TS17: foregut mesentery	Ambiguous

Structure	Level	Pattern	Image	Note
TS17: foregut mesentery	Present			Expression was abnormally high in the foregut mesentery in the heterozygous embryo.
TS17: hindgut mesentery	Strong			Strong expression was noted as in wild type.
TS17: primordial germ cell	Strong			Strong expression was noted as in wild type.
TS17: cardiovascular system mesenchyme	Strong	Regionally restricted		Strong expression was detected in the mesenchyme around the dorsal aorta as in wild type.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present		6A
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	6A	Expression was detected in the developing dermis of the hindlimb.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present		6B	Enhanced expression in the neural tube of the E11.5 heterozygous embryo was similar to that at E10.5.
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	6B	There was enhanced expression in the developing dermis of the hindlimb.

Structure	Level	Pattern	Image	Note
TS19: stomach mesenchyme	Present		6C

Structure	Level	Pattern	Image	Note
TS19: stomach mesenchyme	Present		6D	Enhanced expression was noted in the stomach mesenchyme of the heterozygous embryo.

Structure	Level	Pattern	Image	Note
TS19: dermatome	Present		6E

Structure	Level	Pattern	Image	Note
TS19: dermatome	Present		6F	Enhanced expression in the dermatomes of the E11.5 heterozygous embryo was similar to that at E10.5.
TS19: tail dermatome	Absent		6F	There was no ectopic expression in tail dermatomes.

Structure	Level	Pattern	Image	Note
TS21: dermis	Present	Regionally restricted	7A	Expression was detected in the dermis in the ear.

Structure	Level	Pattern	Image	Note
TS21: dermis	Present	Regionally restricted	7B	Expression was enhanced in the dermis in the ear of the heterozygous embryo.

Structure	Level	Pattern	Image	Note
TS21: dermis	Present		7C
TS21: mammary gland primordium epithelium	Weak		7C

Structure	Level	Pattern	Image	Note
TS21: dermis	Present	Regionally restricted	7D	There was a distinct accumulation of expression in the dermis around the mammary gland in the heterozygous embryo.
TS21: mammary gland primordium epithelium	Weak		7D	Expression was weak in the mammary gland epithelium as in the wild type.

Structure	Level	Pattern	Image	Note
TS21: dermis	Present	Regionally restricted	7E	Expression was detected in the trunk dermis.

Structure	Level	Pattern	Image	Note
TS21: dermis	Present	Regionally restricted	7F	Enhanced expression was detected in the dermis in the trunk.