Nat2 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:127891 Wakefield L, et al., Mouse arylamine N-acetyltransferase 2 (Nat2) expression during embryogenesis: a potential marker for the developing neuroendocrine system. Biomarkers. 2007 Sep 25;:1-13
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:5610025
Gene symbol:	Nat2
Gene name:	N-acetyltransferase 2 (arylamine N-acetyltransferase)

Reporter:	lacZ
	Direct Detection
Assay notes:	Fixative was 2% formaldehyde, 0.2 glutaraldehyde.

Results

Specimen 1A: embryonic day 8.5; Nat2^tm1Esim/Nat2^tm1Esim (more

)

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present		1A
TS13: migrating neural crest	Present	Regionally restricted	1A	Expression is in neural crest cells migrating towards the heart.

Specimen 1B left: embryonic day 10.5; Nat2^tm1Esim/Nat2^tm1Esim (more

)

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present	Regionally restricted	1B left
TS17: hindbrain floor plate	Present		1B left
TS17: nasal pit	Present		1B left
TS17: otocyst	Present		1B left
TS17: peripheral nervous system	Present		1B left
TS17: cranial ganglion	Present	Regionally restricted	1B left	Expression is in the epibranchial placodes.
TS17: optic cup	Present	Regionally restricted	1B left	Expression extends laterally from the optic cup along the ventral surface of the optic stalk.
TS17: optic stalk	Present	Regionally restricted	1B left	Expression extends laterally from the optic cup along the ventral surface of the optic stalk.
TS17: brain floor plate	Present	Regionally restricted	1B left	Expression is anteroposteriorly along the floor plate.
TS17: neural tube floor plate	Present	Regionally restricted	1B left	Expression is anteroposteriorly along the floor plate.

Specimen 1B top right: embryonic day 10.5; Nat2^tm1Esim/Nat2^tm1Esim (more

)

Structure	Level	Pattern	Image	Note
TS17: olfactory epithelium	Present		1B top right

Specimen 1B bottom right: embryonic day 10.5; Nat2^tm1Esim/Nat2^tm1Esim (more

)

Structure	Level	Pattern	Image	Note
TS17: olfactory epithelium	Present		1B bottom right
TS17: optic cup	Present	Regionally restricted	1B bottom right	Expression extends laterally from the optic cup along the ventral surface of the optic stalk.
TS17: optic stalk	Present	Regionally restricted	1B bottom right	Expression extends laterally from the optic cup along the ventral surface of the optic stalk.

Specimen 1C: embryonic day 11.5; Nat2^tm1Esim/Nat2^tm1Esim (more

)

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Present		1C
TS19: peripheral nervous system	Present		1C
TS19: Rathke's pouch	Present	Regionally restricted	1C	Expression is in the hypothalamic-pituitary-adrenal axis, from Rathke's pouch, along the sympathetic chain to the developing urogenital ridge.
TS19: diencephalon	Present	Regionally restricted	1C	Expression is in the hypothalamic-pituitary-adrenal axis, from Rathke's pouch, along the sympathetic chain to the developing urogenital ridge.
TS19: future pituitary	Present	Regionally restricted	1C	Expression is in the hypothalamic-pituitary-adrenal axis, from Rathke's pouch, along the sympathetic chain to the developing urogenital ridge.
TS19: gland	Present	Regionally restricted	1C	Expression is in the hypothalamic-pituitary-adrenal axis, from Rathke's pouch, along the sympathetic chain to the developing urogenital ridge.
TS19: parasympathetic nervous system	Present	Regionally restricted	1C	Expression is in the hypothalamic-pituitary-adrenal axis, from Rathke's pouch, along the sympathetic chain to the developing urogenital ridge.
TS19: urogenital ridge	Present	Regionally restricted	1C	Expression is in the hypothalamic-pituitary-adrenal axis, from Rathke's pouch, along the sympathetic chain to the developing urogenital ridge.

Specimen 1D left: embryonic day 11.5; Nat2^tm1Esim/Nat2^tm1Esim (more

)

Structure	Level	Pattern	Image	Note
TS19: Rathke's pouch	Present		1D left

Specimen 1D middle: embryonic day 11.5; Nat2^tm1Esim/Nat2^tm1Esim (more

)

Structure	Level	Pattern	Image	Note
TS19: sympathetic ganglion	Present		1D middle

Specimen 1D right: embryonic day 11.5; Nat2^tm1Esim/Nat2^tm1Esim (more

)

Structure	Level	Pattern	Image	Note
TS19: sympathetic ganglion	Present		1D right

Specimen 2A: embryonic day 11.5; Nat2^tm1Esim/Nat2^tm1Esim (more

)

Structure	Level	Pattern	Image	Note
TS19: sympathetic ganglion	Present		2A left

Specimen 2A top middle: embryonic day 11.5; Nat2^tm1Esim/Nat2^tm1Esim (more

)

Structure	Level	Pattern	Image	Note
TS19: sympathetic ganglion	Present		2A top middle

Specimen 2A bottom middle: embryonic day 11.5; Nat2^tm1Esim/Nat2^tm1Esim (more

)

Structure	Level	Pattern	Image	Note
TS19: sympathetic ganglion	Present		2A bottom middle

Specimen 2C left: embryonic day 11.5; Nat2^tm1Esim/Nat2^tm1Esim (more

)

Structure	Level	Pattern	Image	Note
TS19: sympathetic ganglion	Present		2C left

Specimen 2C middle: embryonic day 11.5; Nat2^tm1Esim/Nat2^tm1Esim (more

)
Note: Double labeled: blue - Nat2 reporter; red - Nefm (assay MGI:5610096).

Structure	Level	Pattern	Image	Note
TS19: sympathetic ganglion	Present	Regionally restricted	2C middle	Expression is in glial cells.

Specimen 2C right: embryonic day 11.5; Nat2^tm1Esim/Nat2^tm1Esim (more

)
Note: Double labeled: blue - Nat2 reporter; red - Nefm (assay MGI:5610096).

Structure	Level	Pattern	Image	Note
TS19: sympathetic ganglion	Present	Regionally restricted	2C right	Expression is in glial cells.

Specimen 3A: postnatal week 8; Nat2^tm1Esim/Nat2^tm1Esim (more

)

Structure	Level	Pattern	Image	Note
TS28: nervous system	Present	Single cells	3A	Expression is in glial cells interspersed with with atrial myocardial cells.
TS28: ventricle cardiac muscle	Absent

Specimen 3B: postnatal week 8; Nat2^tm1Esim/Nat2^tm1Esim (more

)

Structure	Level	Pattern	Image	Note
TS28: nervous system	Present	Single cells	3B	xpression is in glial cells interspersed with with atrial myocardial cells.

Specimen 3E: postnatal week 8; Nat2^tm1Esim/Nat2^tm1Esim (more

)

Structure	Level	Pattern	Image	Note
TS28: nervous system	Present	Single cells	3E	Expression is in glial cells accompanying the sympathetic nerve along the coronary sinus.
TS28: ventricle cardiac muscle	Absent		3E

Specimen 3F: postnatal week 8; Nat2^tm1Esim/Nat2^tm1Esim (more

)

Structure	Level	Pattern	Image	Note
TS28: nervous system	Present	Single cells	3F	Expression is in glial cells accompanying the sympathetic nerve along the coronary sinus.

Specimen 4C cortex: postnatal adult; Nat2^tm1Esim/Nat2^tm1Esim (more

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Structure	Level	Pattern	Image	Note
TS28: adrenal gland zona fasciculata	Present		4C cortex

Specimen 4C medulla: postnatal adult; Nat2^tm1Esim/Nat2^tm1Esim (more

)

Structure	Level	Pattern	Image	Note
TS28: adrenal medulla	Present	Scattered	4C medulla

Specimen 5A: postnatal adult; Nat2^tm1Esim/Nat2^tm1Esim (more

)

Structure	Level	Pattern	Image	Note
TS28: epithelial layer of oviduct	Strong		5A

Specimen 5C: postnatal adult; Nat2^tm1Esim/Nat2^tm1Esim (more

)
Note: Double labeled: blue - Nat2 reporter; red - Nat2 protein (assay MGI:5610043).

Structure	Level	Pattern	Image	Note
TS28: epithelial layer of oviduct	Strong		5C

Specimen 6A: postnatal adult; Nat2^tm1Esim/Nat2^tm1Esim (more

)
Note: Virgin female.

Structure	Level	Pattern	Image	Note
TS28: mammary gland	Present		6A

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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