Probe:	Atrx cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: gonad	Weak	Diffuse	1A	Expression was diffuse and dispersed throughout the indifferent gonad.
TS19: mesonephros	Weak	Diffuse	1A	To a lesser extent.

Structure	Level	Pattern	Image	Note
TS23: seminiferous cord	Strong		1C
TS23: mesonephros	Absent		1C
TS23: interstitium of the testis	Present	Diffuse	1C

Structure	Level	Pattern	Image	Note
TS24: ovary	Present	Punctate	1E	Expressed in the cortex, indicative of germ cell staining. A low level of diffuse staining persisted throughout the somatic cells.
TS24: mesonephros	Absent		1E

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		4A
TS19: forebrain	Present		4A
TS19: forelimb bud	Present		4A
TS19: hindbrain	Present		4A
TS19: hindlimb bud	Present		4A
TS19: neural tube	Present		4A
TS19: nose	Present		4A
TS19: oral region	Present		4A
TS19: tail	Present		4A
TS19: head	Present	Regionally restricted	4A	Expressed in the craniofacial regions, more intense in the anterior aspects where the nose and mouth are situated.

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		4B
TS19: forelimb bud	Present		4B
TS19: hindlimb bud	Present		4B
TS19: nose	Present		4B
TS19: oral region	Present		4B
TS19: tail	Present		4B

Structure	Level	Pattern	Image	Note
TS20: foot	Present		4C
TS20: forebrain	Present		4C
TS20: hand	Present		4C
TS20: nose	Present		4C
TS20: oral region	Present		4C
TS20: tail	Present		4C

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present		4D
TS20: hindbrain	Present		4D
TS20: spinal cord	Present		4D

Structure	Level	Pattern	Image	Note
TS21: foot	Present		4E
TS21: forebrain	Present		4E
TS21: genital tubercle	Present		4E
TS21: hand	Present		4E
TS21: nose	Present		4E
TS21: oral region	Present		4E

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		4F
TS21: hindbrain	Present		4F
TS21: spinal cord	Present		4F

Structure	Level	Pattern	Image	Note
TS21: genital tubercle of male	Present	Regionally restricted	5A	Expression was more concentrated in the urethral groove on the ventral side.

Structure	Level	Pattern	Image	Note
TS22: genital tubercle of male	Present	Regionally restricted	5B	Expression was more concentrated in the urethral groove on the ventral side.

Structure	Level	Pattern	Image	Note
TS18: gonad primordium	Weak	Diffuse		Expression was diffuse and dispersed throughout the indifferent gonad.
TS18: mesonephros	Weak	Diffuse

Structure	Level	Pattern	Image	Note
TS18: gonad primordium	Weak	Diffuse		Expression was diffuse and dispersed throughout the indifferent gonad.
TS18: mesonephros	Present

Structure	Level	Pattern	Image	Note
TS24: seminiferous cord	Strong
TS24: interstitium of the testis	Present	Diffuse
TS24: mesonephros	Absent

Structure	Level	Pattern	Image	Note
TS17: genital swelling	Present	Regionally restricted		Expression was more concentrated in the urethral groove on the ventral side.

Structure	Level	Pattern	Image	Note
TS24: genital tubercle	Present	Regionally restricted		Expression was more concentrated in the urethral groove on the ventral side.