Wt1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:16791 Rackley RR, et al., Expression of the Wilms' tumor suppressor gene WT1 during mouse embryogenesis. Cell Growth Differ. 1993 Dec;4(12):1023-31
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5604354
Gene symbol:	Wt1
Gene name:	WT1 transcription factor

Antibody:	anti-WT1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to fluorescein

Results

Specimen 3bA: embryonic day 15.5 (more

)

Structure	Level	Image	Note
TS23: nephrogenic interstitium	Present	3bA	Expression in metanephric blastema.
TS23: renal cortex	Present	3bA
TS23: renal vesicle	Present	3bA	Expression in podocyte cells of renal vesicle.
TS23: pretubular aggregate	Present	3bA	Expression in early condensations.

Specimen 3bB: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: renal vesicle	Present		3bB	Expression in podocyte cells of renal vesicle.

Specimen 3bD: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: renal vesicle	Present		3bD	Expression in podocyte cells of renal vesicle.

Specimen 4E: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: seminiferous cord	Present		4E

Specimen 5A: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: paramesonephric duct of female	Strong	Regionally restricted	5A	Strong expression is present in the uterus but not its lining.

Specimen 5C: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: spinal cord mantle layer	Present		5C	Expression in ependymal cells.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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