Probe:	WT1 cDNA6
Probe preparation:	Antisense labelled with I125 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent		1A/B
TS13: extraembryonic component	Absent		1A/B
TS13: trophectoderm	Absent		1A/B
TS13: amnion	Absent		1A/B
TS13: neural ectoderm	Absent		1A/B	No expression in the neuroepithelium of neural tube.
TS13: somite	Absent		1A/B
TS13: pericardial cavity	Absent		1A/B	No expression in pericardioperitoneal cavity.
TS13: peritoneal cavity	Absent		1A/B	No expression in pericardioperitoneal cavity.

Structure	Level	Pattern	Image	Note
TS28: decidua	Strong		1A/B	Strong expression in the maternal endometrium.

Structure	Level	Pattern	Image	Note
TS17: nephric duct, mesonephric portion	Strong		1C/D	Strong expression in mesonephric duct.
TS17: urogenital ridge	Strong		1C/D	Strong expression in urogenital ridge; lower levels in its mesentery.
TS17: nephric duct, metanephric portion	Strong		1C/D	Strong expression in degenerating pronephrons.
TS17: mesothelium	Strong		1C/D	Strong expression in mesothelium of the peritoneal and thoracic cavity, including the thymus, lung and heart.
TS17: dorsal mesogastrium	Present	Regionally restricted	1C/D	Expression in spleen primordium.
TS17: midgut loop dorsal mesentery	Present		1C/D	Expression in midgut with dorsal mesentery.
TS17: 1st branchial arch	Absent		1C/D
TS17: hepatic primordium	Absent		1C/D
TS17: lung	Absent		1C/D
TS17: main bronchus	Absent		1C/D
TS17: pericardial cavity	Absent		1C/D
TS17: stomach lumen	Absent		1C/D
TS17: pancreas primordium	Absent		1C/D

Structure	Level	Pattern	Image	Note
TS19: peripheral blastema	Strong		1E/F	Strong expression in the metanephric blastema.
TS19: dorsal mesogastrium	Strong	Regionally restricted	1E/F	Strong expression in the capsule and epithelial supporting reticular tissue of the spleen primordium.
TS19: urogenital ridge	Strong	Regionally restricted	1E/F	Strong expression in mesentery of urogenital ridge.
TS19: body wall	Strong	Regionally restricted	1E/F	Strong expression in the developing inferoanterior abdominal wall.
TS19: ureteric bud	Absent		1E/F	No expression in ureteric bud and surrounding epithelium.
TS19: iliac artery	Absent		1E/F
TS19: embryo	Present	Regionally restricted	1E/F	Although there is expression elsewhere in the embryo, there is none in the dorsum.

Structure	Level	Pattern	Image	Note
TS19: coelomic epithelium of gonad	Strong		1G/H	Strong expression in germinal epithelium.
TS19: mesonephros	Strong		1G/H	Strong expression in mesonephric vesicle with podocyte cap.
TS19: primordial germ cell of gonad	Absent		1G/H

Structure	Level	Pattern	Image	Note
TS20: metanephros	Strong		2A/B
TS20: testis	Strong		2A/B
TS20: nephric duct of male, mesonephric portion	Strong	Regionally restricted	2A/B	Strong expression supporting stroma of the vas deferens.
TS20: mesothelium	Present		2A/B
TS20: vertebral cartilage condensation	Absent		2A/B	No expression in vertebral bodies.
TS20: liver	Absent		2A/B
TS20: embryo	Present	Regionally restricted	2A/B	Although there is expression elsewhere in the embryo, there is none in the adrenal gland.

Structure	Level	Pattern	Image	Note
TS21: primitive collecting duct	Absent		2C
TS21: renal capsule	Absent		2C
TS21: renal pelvis	Absent		2C

Structure	Level	Pattern	Image	Note
TS22: nephrogenic interstitium	Strong		2E/F	Strong expression in metanephric blastema
TS22: pretubular aggregate	Strong		2E/F	Strong expression in early condensations.
TS22: renal vesicle	Strong		2E/F	Strong expression during podocyte formation.
TS22: renal capsule	Absent		2E/F

Structure	Level	Pattern	Image	Note
TS21: metanephros	Present		4A/B
TS21: testis	Present	Regionally restricted	4A/B	Areas of expression do not include the efferent ductules.
TS21: nephric duct of male, mesonephric portion	Present	Regionally restricted	4A/B	Expression is present in the stroma of vas deferens and epididymis.
TS21: mesothelium	Present		4A/B
TS21: adrenal gland	Absent		4A/B
TS21: pancreas	Absent		4A/B
TS21: stomach epithelium	Absent		4A/B

Structure	Cell Type	Level	Pattern	Image	Note
TS22: Sertoli cell	Sertoli cell	Present		4C/D
TS22: testis		Present	Regionally restricted	4C/D	Areas of expression include the tunica albuginea.
TS22: primary sex cord		Present		4C/D	Expression in seminiferous tubules.
TS22: germ cell of testis		Absent		4C/D	No expression in spermatogonia.

Structure	Level	Pattern	Image	Note
TS22: germ cell of testis	Absent		4F	No expression in spermatogonia.

Structure	Level	Pattern	Image	Note
TS21: lung	Present	Regionally restricted	5B	Expression in the mesothelial lining.
TS21: spinal cord mantle layer	Present	Regionally restricted	5B	Expression in ependymal cells.
TS21: mesothelium	Present		5B
TS21: vertebral cartilage condensation	Absent		5B	No expression in vertebral bodies.

Structure	Level	Pattern	Image	Note
TS21: paramesonephric duct of female	Present	Regionally restricted	5D	Expression is present in the uterus but not its lining.
TS21: mesothelium	Present		5D
TS21: body wall	Present		5D	Expression in abdominal wall musculature.
TS21: midgut	Absent		5D
TS21: primitive bladder	Absent		5D
TS21: rectum	Absent		5D
TS21: stomach epithelium	Absent		5D
TS21: urethra	Absent		5D	No expression in posterior urethra.

Structure	Level	Pattern	Image	Note
TS21: paramesonephric duct of female	Strong	Regionally restricted	5E	Strong expression in the uterine horn.
TS21: metanephros	Present		5E
TS21: spinal cord mantle layer	Present		5E
TS21: surface epithelium of ovary	Present		5E
TS21: ovary	Present	Regionally restricted	5E	Expression present in surface epithelium of ovary and granulosa cells.

Structure	Level	Pattern	Image	Note
TS23: surface epithelium of ovary	Present		5F
TS23: germ cell of ovary	Absent		5F