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Gene Expression Data
Assay Details
Assay
Reference: J:65842 Robb L, et al., Cloning, expression analysis, and chromosomal localization of murine and human homologues of a xenopus mix gene. Dev Dyn. 2000 Dec;219(4):497-504
Assay type: RNA in situ
MGI Accession ID: MGI:5604129
Gene symbol: Mixl1
Gene name: Mix paired-like homeobox
Probe: Mix probe4
Probe preparation: Antisense labelled with digoxigenin RNA
Assay notes: The authors state that similar results were obtained using either of the following probes and did not specify which probe was used: Mix probe2, Mix probe3, and Mix probe4 which are MGI:5603737, MGI:5603738, and MGI:5603739 respectively. Data are annotated to the shortest probe: Mix probe4.
Results
Specimen 5A: embryonic day 5.5 (more )
Structure Level Pattern Image Note
TS8: extraembryonic visceral endoderm Present Regionally restricted 5A Expression is in a radially symmetrical band at the embryonic-extraembryonic junction.
TS8: anterior visceral endoderm Present Patchy 5A
Specimen not shown: embryonic day 5.5 (more )
Structure Level Pattern Image Note
TS8: extraembryonic visceral endoderm Present
Specimen 5B: embryonic day 6.5 (more )
Note: Prestreak embryo.
Structure Level Pattern Image Note
TS9: embryo Present Regionally restricted 5B Expression is on one side of the egg cylinder.
Specimen 5C: embryonic day 6.5 (more )
Note: Prestreak embryo.
Structure Level Pattern Image Note
TS9: embryo Present Regionally restricted 5C Expression is in the posterior epiblast.
TS9: extraembryonic visceral endoderm Present Regionally restricted 5C Expression is in visceral endoderm associated with the primitive streak.
Specimen 5D: embryonic day 6.75 (more )
Note: Early streak embryo.
Structure Level Pattern Image Note
TS10: embryo Present Regionally restricted 5D Expression is in the posterior epiblast.
TS10: extraembryonic visceral endoderm Present Regionally restricted 5D Expression is in visceral endoderm associated with the primitive streak.
Specimen 5E: embryonic day 7.0 (more )
Note: Mid streak embryo.
Structure Level Pattern Image Note
TS10: primitive streak Strong 5E
TS10: embryo mesoderm Strong Regionally restricted 5E Expression is in the nascent mesoderm that spreads over the embryonic and extraembryonic regions.
TS10: extraembryonic mesoderm Strong Regionally restricted 5E Expression is in the nascent mesoderm that spreads over the embryonic and extraembryonic regions.
Specimen 5F: embryonic day 7.25 (more )
Note: Late streak embryo.
Structure Level Pattern Image Note
TS10: primitive streak Strong 5F
TS10: embryo mesoderm Present 5F Expression is in the nascent mesoderm that spreads over the embryonic and extraembryonic regions.
TS10: extraembryonic mesoderm Present 5F Expression is in the nascent mesoderm that spreads over the embryonic and extraembryonic regions.
Specimen 5G: embryonic day 7.25 (more )
Note: No allantoic bud stage embryo.
Structure Level Pattern Image Note
TS10: primitive streak Strong Graded 5G Expression is strongest in the proximal two-thirds of the streak.
TS10: allantois Weak 5G
TS10: node Absent 5G
Specimen 5H: embryonic day 7.25 (more )
Note: Early bud stage embryo.
Structure Level Pattern Image Note
TS10: primitive streak Strong Graded 5H Expression is strongest in the proximal two-thirds of the streak.
TS10: allantois Weak 5H
TS10: node Absent 5H
Specimen 5I: embryonic day 7.5 (more )
Note: Late bud stage embryo.
Structure Level Pattern Image Note
TS11: primitive streak Strong Graded 5I Expression is strongest in the proximal two-thirds of the streak.
TS11: allantois Weak 5I
TS11: node Absent 5I
Specimen 5J: embryonic day 7.75 (more )
Note: Late headfold stage emrbyo.
Structure Level Pattern Image Note
TS12: primitive streak Present Regionally restricted 5J Expression is in the posterior end.
Specimen 5K: embryonic day 7.75 (more )
Note: Late headfold stage emrbyo.
Structure Level Pattern Image Note
TS12: primitive streak Present Regionally restricted 5K Expression is in the posterior end.
Specimen 5L: embryonic day 7.75 (more )
Note: Late headfold stage emrbyo.
Structure Level Pattern Image Note
TS12: primitive streak Present Regionally restricted 5L Expression is in the posterior end.
Specimen 5M: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: tail Present 5M The authors state that expression was absent after E9.5.
Specimen 5N: embryonic day 6.75 (more )
Note: Early streak embryo.
Structure Level Pattern Image Note
TS10: embryo Present Regionally restricted 5N Expression is in the posterior epiblast.
TS10: extraembryonic visceral endoderm Present Regionally restricted 5N Expression is in visceral endoderm associated with the primitive streak.
Specimen 5O: embryonic day 6.75 (more )
Note: Early streak embryo.
Structure Level Pattern Image Note
TS10: embryo Present Regionally restricted 5O Expression is in the posterior epiblast.
TS10: extraembryonic visceral endoderm Present Regionally restricted 5O Expression is in visceral endoderm associated with the primitive streak.
Specimen 5P: embryonic day 7.0 (more )
Note: Mid streak embryo.
Structure Level Pattern Image Note
TS10: primitive streak Strong 5P
TS10: embryo mesoderm Present Regionally restricted 5P Expression is in the nascent mesoderm that spreads over the embryonic and extraembryonic regions.
TS10: extraembryonic mesoderm Present Regionally restricted 5P Expression is in the nascent mesoderm that spreads over the embryonic and extraembryonic regions.
Specimen 5Q: embryonic day 8.0 (more )
Note: 4 somites.
Structure Level Pattern Image Note
TS12: notochord Present Single cells 5Q Expression is in crown cells of the caudal notochord.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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