Probe:	Clone 19/17
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	Two probes were described, but the probe used in this assay was not detailed - see also MGI:5604011. The results obtained with the two probes were similar.

Structure	Level	Pattern	Image	Note
TS12: embryo	Weak	Widespread	2a
TS12: allantois	Strong		2a
TS12: future prosencephalon	Strong		2a
TS12: eye	Strong	Regionally restricted	2a	Expression was stronger in the forming optic placodes.
TS12: amnion	Absent		2a
TS12: heart	Absent		2a

Structure	Level	Pattern	Image	Note
TS14: embryo	Present	Widespread	2b
TS14: future midbrain	Strong	Regionally restricted	2b	Expression was detected in the ventral midbrain.
TS14: future telencephalon	Strong		2b	Expression was detected in telencephalic neural tissues surrounding the optic vesicle.
TS14: 1st branchial arch	Strong		2b
TS14: future brain	Strong	Regionally restricted	2b	Expression was detected in the trunk and head ventral neural tube.
TS14: neural tube	Strong	Regionally restricted	2b	Expression was detected in the trunk and head ventral neural tube.
TS14: heart	Absent		2b

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Widespread	2c
TS15: future brain	Strong	Regionally restricted	2c	Expression was detected in the ventral neural tube.
TS15: neural tube	Strong	Regionally restricted	2c	Expression was detected in the ventral neural tube.
TS15: telencephalon	Strong	Regionally restricted	2c	Expression was detected in telencephalic neural tissues surrounding the optic vesicle.
TS15: 1st branchial arch	Strong		2c
TS15: forelimb bud mesenchyme	Strong		2c
TS15: somite	Strong		2c
TS15: heart	Absent		2c

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted	2d	Expression was detected in the ventral midbrain.
TS17: lens pit	Present		2d
TS17: trunk mesenchyme	Present	Regionally restricted	2d	Expressing cells migrating from the somites were positive and extended toward the ventral side of the embryo.
TS17: limb mesenchyme	Present	Regionally restricted	2d	Expression was less prominent and more restricted to internal mesenchyme.
TS17: heart	Absent		2d

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Widespread	2e
TS19: lens vesicle	Strong		2e
TS19: forelimb bud	Present		2e
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	2e	Expression was detected in the hindlimb mesoderm.
TS19: trunk mesenchyme	Present	Regionally restricted	2e	Expression was positive in somite derivatives.

Structure	Level	Pattern	Image	Note
TS16: lens pit	Present		2e eye E10

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Present		2e E11.5

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Present		2e E12

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Present	Regionally restricted	2e E12.5	Expression was downregulated in the lens center.

Structure	Level	Pattern	Image	Note
TS20: forelimb interdigital region	Present
TS20: hand mesenchyme	Present	Regionally restricted		Expression was detected in the perichondrial mesenchyme.
TS20: hand pre-cartilage condensation	Absent			Expression was absent from the condensing cartilage in undifferentiated digital rays.

Structure	Level	Pattern	Image	Note
TS21: central nervous system	Strong		2f
TS21: hand	Strong		2f
TS21: blood vessel	Absent		2f
TS21: eye	Absent		2f

Structure	Level	Pattern	Image	Note
TS22: foot	Present		2g
TS22: spinal cord	Present		2g

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Strong	Regionally restricted	2h	Expression was strong in the perichondrial mesenchyme.
TS21: forelimb interphalangeal joint	Present	Regionally restricted	2h	Expression was upregulated in the most proximal phalanx joints of all digits.

Structure	Level	Pattern	Image	Note
TS22: forelimb interphalangeal joint	Present	Regionally restricted	2i	Expression was detected in the proximal and a second row of phalanx joints of all digits, except the first digit where only one joint was visible.

Structure	Level	Pattern	Image	Note
TS19: gonad	Weak	Uniform

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present		2j
TS22: foot	Present		2j
TS22: genital tubercle	Present		2j
TS22: gonad	Present		2j
TS22: mesenchyme of metanephros	Present		2j
TS22: spinal cord	Present		2j

Structure	Level	Pattern	Image	Note
TS21: ovary	Present	Clusters	2k

Structure	Level	Pattern	Image	Note
TS21: ovary	Present	Regionally restricted	2l	The cluster distribution was stronger in the outer layer. Expression was always absent in interstitial cells.
TS21: germ cell of ovary	Present		2l	Clumps of positive cells correspond to the germ cells.
TS21: mesonephric mesenchyme of female	Present	Regionally restricted	2l	Positive mesonephros mesenchyme surrounded the Mullerian duct.

Structure	Level	Pattern	Image	Note
TS21: primary sex cord	Present		2m

Structure	Cell Type	Level	Pattern	Image	Note
TS21: germ cell of testis		Present		2n
TS21: primary sex cord		Present		2n
TS21: Sertoli cell	Sertoli cell	Absent		2n
TS21: mesonephric mesenchyme of male		Present	Regionally restricted	2n	Positive mesonephros mesenchyme surrounded the paramesonephric duct.