Antibody:	anti-Tuj1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS26: carotid body	Present		7A	Expressed in the sympathetic neurons.
TS26: superior cervical ganglion	Present		7A	Expressed in the sympathetic neurons.
TS26: cranial nerve	Present	Regionally restricted	7A	Expressed in the carotid sinus nerve, which was densely distributed around the root of the internal carotid artery.

Structure	Level	Pattern	Image	Note
TS26: carotid body	Present		7B	Expressed in the sympathetic neurons.
TS26: superior cervical ganglion	Present		7B	Expressed in the sympathetic neurons.
TS26: cranial nerve	Present	Regionally restricted	7B	Expressed in the carotid sinus nerve, which was densely distributed around the root of the internal carotid artery.

Structure	Level	Pattern	Image	Note
TS21: superior cervical ganglion	Present
TS21: common carotid artery	Present	Regionally restricted		Expresse in glomus cells in the peripheral portion of the carotid body rudiment in the carotid artery bifurcation.

Structure	Level	Pattern	Image	Note
TS21: superior cervical ganglion	Strong			Intense expression in the sympathetic neurons in the superior cervical ganglion, which had an attenuated growth relative to that of the wild-type.