Probe:	Reelin cDNA2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS22: embryo	Present		1A	Expression was detected in many regions.

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Weak	Widespread	2A	Expression was weak throughout the neuroepithelium of the telencephalon, but expression was slightly stronger at the rostral and caudal ends.
TS15: diencephalon	Weak	Regionally restricted	2A
TS15: future midbrain	Weak	Regionally restricted	2A	Expression was detected along the marginal zone.
TS15: future hindbrain	Weak	Regionally restricted	2A	Weak expression was detected at the caudal end of the hindbrain.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex marginal layer	Present		2B
TS20: neocortex	Present		2B
TS20: midbrain	Present	Regionally restricted	2B	Expression was detected in the tectum and the tegmentum.
TS20: cerebellum primordium	Present		2B
TS20: medulla oblongata	Present		2B
TS20: pons	Present		2B
TS20: remnant of Rathke's pouch	Present		2B
TS20: ganglionic eminence	Absent		2B
TS20: hypothalamus	Absent		2B	Expression was not detected in the preoptic region.

Structure	Level	Pattern	Image	Note
TS22: brain	Strong	Regionally restricted	2C	Strong expression was maintained in the same regions as in earlier stages.
TS22: cerebellum	Strong		2C
TS22: cerebral cortex	Strong		2C
TS22: hippocampus	Strong		2C
TS22: main olfactory bulb	Strong		2C
TS22: tectum	Strong		2C
TS22: medullary velum	Strong		2C
TS22: brainstem	Present	Regionally restricted	2C	Expression was detected in the inferior olive.
TS22: thalamus	Present	Regionally restricted	2C	Expression was detected in the anterior and intermediate thalamus.
TS22: hypothalamus	Present	Regionally restricted	2C	Expression was detected in the anterior hypothalamus and preoptic region.

Structure	Level	Pattern	Image	Note
TS24: brain	Present	Regionally restricted	2D	The expression pattern was similar to that observed at E14.5.
TS24: cerebral cortex	Strong		2D
TS24: inferior colliculus	Strong		2D	Signal intensity was much higher in the inferior colliculus than in the superior colliculus.
TS24: superior colliculus	Present		2D	Signal intensity was much higher in the inferior colliculus than in the superior colliculus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex marginal layer	Strong		2G/G' , 2E
TS24: cortical plate	Weak	Single cells	2G/G' , 2E
TS24: cortical subplate	Weak	Single cells	2G/G' , 2E
TS24: hippocampus	Present		2E

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Absent		2F
TS24: hippocampus	Absent		2F

Structure	Level	Pattern	Image	Note
TS13: blood island	Present		3A/A'
TS13: foregut diverticulum	Present		3A/A'
TS13: neural tube	Present		3A/A'
TS13: somite	Present		3A/A'

Structure	Level	Pattern	Image	Note
TS13: blood island	Present		3B/B'
TS13: foregut diverticulum	Present		3B/B'
TS13: neural tube	Present		3B/B'

Structure	Level	Pattern	Image	Note
TS15: optic cup	Present		4A/A'
TS15: somite	Present		4A/A'

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present	Regionally restricted	4B/B'	Expression was detected at the cervical level of the spinal cord but not in the caudal region.
TS15: future brain	Present		4B/B'
TS15: somite	Present		4B/B'
TS15: trigeminal preganglion	Present		4B/B'
TS15: hepatic primordium	Strong		4B/B'

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present		4C/C'
TS15: future brain	Present		4C/C'

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present	Regionally restricted	4D/D'	Expression was detected at the cervical level of the spinal cord but not in the caudal region.
TS15: hepatic primordium	Strong		4D/D'
TS15: somite	Present		4D/D'

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present		5A/A'
TS20: telencephalon	Present		5A/A'
TS20: vomeronasal organ	Present		5A/A'
TS20: embryo mesenchyme	Present	Regionally restricted	5A/A'	Expression was detected in the condensed mesenchyme surrounding small developing blood vessels.
TS20: Meckel's cartilage	Present	Regionally restricted	5A/A'	Expression was detected in the precartilage primordium.
TS20: mandibular process mesenchyme	Present	Regionally restricted	5A/A'	Expression was detected in the precartilage primordium.
TS20: maxillary process mesenchyme	Present	Regionally restricted	5A/A'	Expression was detected in the precartilage primordium.
TS20: nasal septum mesenchyme	Present	Regionally restricted	5A/A'	Expression was detected in the precartilage primordium.
TS20: temporal bone	Present	Regionally restricted	5A/A'	Expression was detected in the precartilage primordium.
TS20: limb pre-cartilage condensation	Present		5A/A'
TS20: cranial nerve	Present	Regionally restricted	5A/A'	Expression was detected along the olfactory and optic nerve fibers.
TS20: spinal cord	Strong		5A/A'	Expression was strong at both the cervical and thoracic levels.
TS20: somite	Present		5A/A'
TS20: spinal cord mantle layer	Present		5A/A'
TS20: basal columns	Present	Regionally restricted	5A/A'	Expression was detected in part of the ependymal layer in the basal plate.
TS20: alar columns	Weak		5A/A'

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present		5B/B'
TS20: liver	Present		5B/B'
TS20: somite	Present		5B/B'
TS20: stomach epithelium	Present		5B/B'
TS20: spinal cord	Strong		5B/B'	Expression was strong in the spinal cord at both the cervical and thoracic levels.
TS20: basal columns	Present	Regionally restricted	5B/B'	Expression was detected in part of the ependymal layer in the basal plate.
TS20: alar columns	Weak		5B/B'

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Present		6A

Structure	Level	Pattern	Image	Note
TS24: liver	Present		6B

Structure	Level	Pattern	Image	Note
TS24: metanephros	Present		6D

Structure	Level	Pattern	Image	Note
TS22: pituitary gland	Present		7A
TS22: blood vessel	Present	Regionally restricted	7A	Expression was detected in endothelial cells surrounding small blood vessels along the body wall.

Structure	Level	Pattern	Image	Note
TS22: adrenal medulla	Present
TS22: diaphragm	Present
TS22: gut mesentery	Present
TS22: heart	Present
TS22: lung	Present
TS22: testis	Present

Structure	Level	Pattern	Image	Note
TS22: vibrissa dermal component	Present	Regionally restricted	7B	Expression was detected in the dermal papilla of the vibrissae.
TS22: tooth	Present		7B

Structure	Level	Pattern	Image	Note
TS22: blood vessel	Present	Regionally restricted	7C	Expression was detected in endothelial cells surrounding small blood vessels along the body wall.
TS22: dental papilla	Present		7C
TS22: tooth epithelium	Present		7C

Structure	Level	Pattern	Image	Note
TS22: olfactory nerve	Present		7D
TS22: vomeronasal organ	Present		7D

Structure	Level	Pattern	Image	Note
TS22: olfactory nerve	Present		7E , 7F

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Widespread	8C/C' , 8A/A'	Expression was detected in all cortical layers 1-6.
TS28: hippocampus	Present	Non-Uniform	8A/A'	Expression was detected mostly in neurons in the superficial layer with some detected in deeper layers.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Absent		8B/B'
TS28: hippocampus	Absent		8B/B'

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Weak		9A/A'

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	Strong		9B/B'
TS28: main olfactory bulb glomerular layer	Strong		9B/B'	Strong expression was noted in periglomerular cells.

Structure	Level	Pattern	Image	Note
TS28: spinal cord dorsal horn	Present	Regionally restricted	9C/C'	Expression was localized to neurons in the outer lining of the dorsal horn.
TS28: spinal cord ventral horn	Present		9C/C'

Structure	Level	Pattern	Image	Note
TS28: liver vascular element	Present	Regionally restricted	9D/D'	Expression was restricted to endothelial cells along the sinusoidal capillaries and was not detected in the hepatic cells.

Structure	Level	Pattern	Image	Note
TS28: renal vasculature	Present	Regionally restricted	9E/E'	Expression was detected by some endothelial cells along blood vessels.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Weak		9F/F'

Structure	Level	Pattern	Image	Note
TS28: ovary	Present	Regionally restricted	9G/G'	Expression was localized to the interstitial region.
TS28: ovary follicle	Absent		9G/G'

Structure	Level	Pattern	Image	Note
TS28: spleen	Trace	Regionally restricted		Very weak expression was detected in some endothelial cells.
TS28: heart	Absent
TS28: intestine	Absent
TS28: lung	Absent
TS28: thymus	Absent