Tg(Csf1r*-GAL4/VP16,UAS-ECFP)1Hume
Transgene Detail
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| Symbol: |
Tg(Csf1r*-GAL4/VP16,UAS-ECFP)1Hume |
| Name: |
transgene insertion 1, David A Hume |
| MGI ID: |
MGI:5587942 |
| Synonyms: |
Csf1r-Gal4VP16/UAS-ECFP, MacBlue |
| Transgene: |
Tg(Csf1r*-GAL4/VP16,UAS-ECFP)1Hume Location: unknown
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| Alliance: |
Tg(Csf1r*-GAL4/VP16,UAS-ECFP)1Hume page
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| Strain of Origin: |
(C57BL/6J x CBA)F2
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| Transgene Type: |
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Transgenic (Reporter) |
| Mutation: |
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Insertion
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Mutation details: Two transgenes inserted at a single locus. One transgenic construct is designed with a colony stimulating factor 1 receptor (Csf1r) promoter, lacking the 158 bp (-454 to -298 relative to ATG) trophoblast transcription start sites and a TATA-box. This mutated Csf1r drives expression of a "Gal4 driver" (containing a yeast GAL4 transcriptional activator fused to herpes simplex virus protein (VP16)). A "Gal4-responder" plasmid is designed to contain an enhanced cyan fluorescent protein (ECFP) uder the control of a yeast upstream activating sequence (UAS) Gal4-dependent promoter. The founder line with approximately 5% ECFP+ leukocytes was selected for characterization.
(J:131223)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 2 strains available
Cell Lines: 0 lines available
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| Original: |
J:131223 Ovchinnikov DA, et al., Expression of Gal4-dependent transgenes in cells of the mononuclear phagocyte system labeled with enhanced cyan fluorescent protein using Csf1r-Gal4VP16/UAS-ECFP double-transgenic mice. J Leukoc Biol. 2008 Feb;83(2):430-3 |
| All: |
20 reference(s) |
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Analysis Tools
