Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(CAG-RFP*,-Dscam,-EGFP)1Pfu
Name:	transgene insertion 1, Peter Fuerst
MGI ID:	MGI:5584100
Synonyms:	DscamfloxGOF
Transgene:	Tg(CAG-RFP*,-Dscam,-EGFP)1Pfu  Location: unknown
Alliance:	Tg(CAG-RFP*,-Dscam,-EGFP)1Pfu page

Strain of Origin:

(C57BL/6 x SJL)F1

Transgene Type:    Transgenic (Conditional ready, Inserted expressed sequence, Reporter) Mutation:    Insertion   Tg(CAG-RFP*,-Dscam,-EGFP)1Pfu expresses 1 gene Transgene expresses: Organism Expressed Gene Note mouse Dscam (MGI:1196281)   Mutation details: This transgene contains (from 5' to 3') the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from the pCAGGS vector), a loxP site, the cytoplasmic red fluorescent protein tdimer2(12) (a non-oligomerizing DsRed variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), a polyadenylation signal, a second loxP site, a full-length mouse Down syndrome cell adhesion molecule (Dscam) sequence including the endogenous stop codon, an internal ribosome entry site (IRES), an enhanced green fluorescent protein sequence and a polyadenylation signal. Red fluorescence protein is expressed in all cells/tissues examined. Cre recombinase exposure deletes the floxed tdimer2(12) sequence and results in expression of full-length Dscam and EGFP fluorescence. (J:101977, J:219627)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:219627 Li S, et al., DSCAM Promotes Refinement in the Mouse Retina through Cell Death and Restriction of Exploring Dendrites. J Neurosci. 2015 Apr 8;35(14):5640-54
All:	5 reference(s)