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Gene Expression Data
Assay Details
Assay
Reference: J:148405 Nel-Themaat L, et al., Morphometric analysis of testis cord formation in Sox9-EGFP mice. Dev Dyn. 2009 May;238(5):1100-10
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5581626
Gene symbol: Sox9
Gene name: SRY (sex determining region Y)-box 9
Reporter: GFP
Direct Detection
Results
Specimen 1A: embryonic day 11.5; Sox9tm1Haak/Sox9+ (more )
Note: tail somite stage 19
Structure Level Pattern Image Note
TS19: gonad Present Regionally restricted 1A Expressed in the Sertoli and germ cell mass. Surrounded by a layer of enhanced green fluorescent protein (EGFP)-negative tissue.

Specimen 1B: embryonic day 12.5; Sox9tm1Haak/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS20: germ cell of testis Strong Regionally restricted 1B Expressed along the entire length of the gonad. Expressed in the Sertoli and germ cell mass. Surrounded by a layer of enhanced green fluorescent protein (EGFP)-negative tissue.
TS20: testis Strong Regionally restricted 1B Expressed along the entire length of the gonad. Expressed in the Sertoli and germ cell mass. Surrounded by a layer of enhanced green fluorescent protein (EGFP)-negative tissue.
TS20: Sertoli cell Sertoli cell Strong Regionally restricted 1B

Specimen 1C: embryonic day 14.5; Sox9tm1Haak/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS22: germ cell of testis Strong Regionally restricted 1C Expressed along the entire length of the gonad. Expressed in the Sertoli and germ cell mass. Surrounded by a layer of enhanced green fluorescent protein (EGFP)-negative tissue.
TS22: testis Strong Regionally restricted 1C Expressed along the entire length of the gonad. Expressed in the Sertoli and germ cell mass. Surrounded by a layer of enhanced green fluorescent protein (EGFP)-negative tissue.
TS22: Sertoli cell Sertoli cell Strong 1C

Specimen 1D: embryonic day 15.5; Sox9tm1Haak/Sox9+ (more )
Structure Level Pattern Image Note
TS23: epididymis Present 1D
TS23: testis Present 1D

Specimen 1E: embryonic day 16.5; Sox9tm1Haak/Sox9+ (more )
Structure Level Pattern Image Note
TS24: testis Strong 1E
TS24: epididymis Present 1E

Specimen 1F: embryonic day 16.5; Sox9tm1Haak/Sox9+ (more )
Structure Level Pattern Image Note
TS24: testis Strong 1F
TS24: ductus deferens Present 1F Expressed in the Wolffian duct.

Specimen 2B/C/D: embryonic day 13.5; Sox9tm1Haak/Sox9+ (more )
Note: Double labeled: green - Sox9 reporter; red - Sox9 protein (assay MGI:5581710).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present 2B , 2C/D Expressed in the cytoplasm.

Specimen 2F/G/H: embryonic day 13.5; Sox9tm1Haak/Sox9+ (more )
Note: Double labeled: green - Sox9 reporter; red - Sox9 protein (assay MGI:5581710).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present 2F , 2G/H Expressed in the cytoplasm.

Specimen 4A: embryonic day 11.5; Sox9tm1Haak/Sox9+ (more )
Note: tail somite stage 19
Structure Level Pattern Image Note
TS19: gonad Present Regionally restricted 4A Expressed in Sertoli cells.

Specimen 4B: embryonic day 11.8; Sox9tm1Haak/Sox9+ (more )
Note: tail somite stage 21
Structure Level Pattern Image Note
TS19: gonad Present Regionally restricted 4B Expressed in Sertoli cells.

Specimen 4C: embryonic day 12.1; Sox9tm1Haak/Sox9+ (more )
Note: tail somite stage 24
Structure Cell Type Level Pattern Image Note
TS20: Sertoli cell Sertoli cell Present 4C

Specimen 4D: embryonic day 12.5; Sox9tm1Haak/Sox9+ (more )
Note: tail somite stage 32
Structure Cell Type Level Pattern Image Note
TS20: Sertoli cell Sertoli cell Present 4D

Specimen 4E: embryonic day 14.5; Sox9tm1Haak/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS22: Sertoli cell Sertoli cell Present 4E

Specimen 5B: embryonic day 14.5; Sox9tm1Haak/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS22: Sertoli cell Sertoli cell Present 5B

Specimen 5C: embryonic day 14.5; Sox9tm1Haak/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS22: Sertoli cell Sertoli cell Present 5C

Specimen 7A: embryonic day 14.5; Sox9tm1Haak/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS22: Sertoli cell Sertoli cell Present 7A

Specimen 7B: embryonic day 14.5; Sox9tm1Haak/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS22: Sertoli cell Sertoli cell Present 7B

Specimen 7C: embryonic day 14.5; Sox9tm1Haak/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS22: Sertoli cell Sertoli cell Present 7C

Specimen 7D: embryonic day 14.5; Sox9tm1Haak/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS22: Sertoli cell Sertoli cell Present 7D

Specimen not shown: embryonic day 10.5; Sox9tm1Haak/Sox9+ (more )
Structure Level Pattern Image Note
TS17: gonad primordium Absent

Specimen not shown: embryonic day 10.5; Sox9tm1Haak/Sox9+ (more )
Structure Level Pattern Image Note
TS17: gonad primordium Absent

Specimen not shown: embryonic day 12.0; Sox9tm1Haak/Sox9+ (more )
Note: tail somite stage 22
Structure Level Pattern Image Note
TS20: testis Strong Widespread Expressed along the entire length of the gonad.

Specimen not shown: embryonic day 12.5; Sox9tm1Haak/Sox9+ (more )
Structure Level Pattern Image Note
TS20: ovary Absent

Specimen not shown: embryonic day 14.5; Sox9tm1Haak/Sox9+ (more )
Structure Level Pattern Image Note
TS22: ovary Absent
TS22: paramesonephric duct of female Present Expressed in the Mullerian duct.

Specimen not shown: embryonic day 15.5; Sox9tm1Haak/Sox9+ (more )
Structure Level Pattern Image Note
TS23: ovary Absent
TS23: paramesonephric duct of female Present Expressed in the Mullerian duct.

Specimen not shown: embryonic day 16.5; Sox9tm1Haak/Sox9+ (more )
Structure Level Pattern Image Note
TS24: ovary Absent
TS24: paramesonephric duct Present Expressed in the Mullerian duct.

Specimen not shown: postnatal adult; Sox9tm1Haak/Sox9+ (more )
Structure Level Pattern Image Note
TS28: seminiferous tubule Strong


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory