Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(DMD)72Thoen
Name:	transgene insertion 72, Peter 't Hoen
MGI ID:	MGI:5578554
Synonyms:	hDMD
Transgene:	Tg(DMD)72Thoen  Location: unknown  Genetic Position: Chr5, Syntenic
Alliance:	Tg(DMD)72Thoen page

Germline Transmission:	Earliest citation of germline transmission: J:132320
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	(DBA/2 x 129P2/OlaHsd)F1

Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence) Mutation:    Insertion   Tg(DMD)72Thoen expresses 1 gene Transgene expresses: Organism Expressed Gene Homolog in Mouse Note human DMD (1756) Dmd  (MGI:94909)   Mutation details: The construct consists of a 2.7 Mbp yeast artificial chromosome (YAC) sequence containing the full-length 2.3 Mbp human dystrophin gene (DMD) and a hygromycin resistance cassette on one end and a neomycin resistance cassette on the other. Spheroplast fusion of YAC-containing yeast cells with murine (DBA2 x 129OLA)F1-derived embryonic stem (ES) cells resulted in ES cells with the integration of the transgene as a tail to tail duplication into mouse chromosome 5. Analysis by Western blot and human-specific antibody demonstrated the presence of full length protein at the sarcolemma. (J:132320)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 3 strains available      Cell Lines: 0 lines available

Original:	J:132320 't Hoen PA, et al., Generation and characterization of transgenic mice with the full-length human DMD gene. J Biol Chem. 2008 Feb 29;283(9):5899-907
All:	6 reference(s)