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Gene Expression Data
Assay Details
Assay
Reference: J:162870 Bax NA, et al., Cardiac malformations in Pdgfralpha mutant embryos are associated with increased expression of WT1 and Nkx2.5 in the second heart field. Dev Dyn. 2010 Aug;239(8):2307-17
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5576299
Gene symbol: Nkx2-5
Gene name: NK2 homeobox 5
Antibody: anti-Nkx-2.5 (N-19): sc-8697
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Results
Specimen 4e: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: heart Ambiguous 4e Almost no expression.
Specimen 4f: embryonic day 13.5; Pdgfratm1Sor/Pdgfratm1Sor (more )
Note: It is possible the specimen carries the Pdgfratm2Sor allele.
Structure Level Pattern Image Note
TS21: heart Present 4f More positive cells in the sinoatrial node and the myocardium of the cardinal veins, compared to the wildtype.
TS21: cardiac muscle tissue Present Regionally restricted 4f More positive cells in the myocardium of the cardinal veins, compared to the wildtype.
Specimen S5c: embryonic day 14.5; Pdgfratm11(EGFP)Sor/Pdgfratm11(EGFP)Sor (more )
Structure Level Pattern Image Note
TS22: cardiac muscle tissue Present Regionally restricted S5c Expressed in the myocardium of the pulmonary vein.
TS22: pulmonary trunk Present Regionally restricted S5c Expressed in the myocardium of the pulmonary vein.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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