Tg(Ube3a)1Mpan
Transgene Detail
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| Symbol: |
Tg(Ube3a)1Mpan |
| Name: |
transgene insertion 1, Matthew Anderson |
| MGI ID: |
MGI:5576240 |
| Synonyms: |
FLAG-Ube3a |
| Transgene: |
Tg(Ube3a)1Mpan Location: unknown
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| Alliance: |
Tg(Ube3a)1Mpan page
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| Transgene Type: |
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Transgenic (Epitope tag, Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(Ube3a)1Mpan expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Note |
| mouse |
Ube3a (MGI:105098) |
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Mutation details: The bacterial artificial chromosome (BAC) clone RP24-178G7 contains the entire ubiquitin protein ligase E3A locus (Ube3a) as well as ~63 kbp of 5' flanking sequence and ~21 kbp of 3' flanking sequence. DNA sequences encoding three FLAG epitope tags and two translational stop codons were inserted in-frame at the 3' coding/untranslated boundary of exon 12 of the gene on the BAC via homologous recombination/BAC recombineering. A single frt site remains just downstream of the 3xFLAG region. Two founder lines with independent integration sites were established; lines 1 and 2. Both founder lines exhibit the same expression pattern in the tissues examined (although line 2 may have slightly lower levels). Animals from line 1 were more extensively characterized. Ube3a expression was confirmed by Western blot of cortical lysates.
(J:178319)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 2 strains available
Cell Lines: 0 lines available
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| Original: |
J:178319 Smith SE, et al., Increased gene dosage of Ube3a results in autism traits and decreased glutamate synaptic transmission in mice. Sci Transl Med. 2011 Oct 5;3(103):103ra97 |
| All: |
10 reference(s) |
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Analysis Tools
