Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Ube3a)1Mpan
Name:	transgene insertion 1, Matthew Anderson
MGI ID:	MGI:5576240
Synonyms:	FLAG-Ube3a
Transgene:	Tg(Ube3a)1Mpan  Location: unknown
Alliance:	Tg(Ube3a)1Mpan page

Strain of Origin:

FVB/NJ

Transgene Type:    Transgenic (Epitope tag, Inserted expressed sequence) Mutation:    Insertion   Tg(Ube3a)1Mpan expresses 1 gene Transgene expresses: Organism Expressed Gene Note mouse Ube3a (MGI:105098)   Mutation details: The bacterial artificial chromosome (BAC) clone RP24-178G7 contains the entire ubiquitin protein ligase E3A locus (Ube3a) as well as ~63 kbp of 5' flanking sequence and ~21 kbp of 3' flanking sequence. DNA sequences encoding three FLAG epitope tags and two translational stop codons were inserted in-frame at the 3' coding/untranslated boundary of exon 12 of the gene on the BAC via homologous recombination/BAC recombineering. A single frt site remains just downstream of the 3xFLAG region. Two founder lines with independent integration sites were established; lines 1 and 2. Both founder lines exhibit the same expression pattern in the tissues examined (although line 2 may have slightly lower levels). Animals from line 1 were more extensively characterized. Ube3a expression was confirmed by Western blot of cortical lysates. (J:178319)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available

Original:	J:178319 Smith SE, et al., Increased gene dosage of Ube3a results in autism traits and decreased glutamate synaptic transmission in mice. Sci Transl Med. 2011 Oct 5;3(103):103ra97
All:	8 reference(s)