Tg(Prnp-Immt/SOD1)1Gmnf
Transgene Detail
|
Symbol: |
Tg(Prnp-Immt/SOD1)1Gmnf |
Name: |
transgene insertion 1, Giovanni Manfredi |
MGI ID: |
MGI:5575877 |
Synonyms: |
mito-SOD1WT, mito-WTSOD1, Prp-mito-WTSOD1 |
Transgene: |
Tg(Prnp-Immt/SOD1)1Gmnf Location: unknown
|
Alliance: |
Tg(Prnp-Immt/SOD1)1Gmnf page
|
|
|
Transgene Type: |
|
Transgenic (Humanized sequence, Inserted expressed sequence) |
Mutation: |
|
Insertion
|
|
|
Tg(Prnp-Immt/SOD1)1Gmnf expresses
1 gene
Transgene expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
SOD1 (6647) |
|
|
|
|
|
Mutation details: The mito-WTSOD1 fusion protein contains a mouse mitofilin (Immt) cDNA sequence encoding the first 187 amino acids (including the mitochondrial targeting presequence, MMP cleavage site and transmembrane domain) fused in-frame to the N-terminus of a wild-type human Cu,Zn superoxide dismutase (SOD1) cDNA sequence. The SOD1 start codon was removed and substituted with an in-frame 6 nt DNA linker. This mito-WTSOD1 sequence was inserted between exon 2 and exon 3 of mouse prion protein (PrP or Prnp) gene at a unique XhoI site in the MoPrP.Xho plasmid vector. The founder line with the highest expression level (founder line 1) was characterized.
(J:211695)
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
|
|
Original: |
J:211695 Fischer LR, et al., SOD1 targeted to the mitochondrial intermembrane space prevents motor neuropathy in the Sod1 knockout mouse. Brain. 2011 Jan;134(Pt 1):196-209 |
All: |
4 reference(s) |
|