Tg(Tagln-PLA2G2A)BMrj
Transgene Detail
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| Symbol: |
Tg(Tagln-PLA2G2A)BMrj |
| Name: |
transgene insertion B, Michel Raymondjean |
| MGI ID: |
MGI:5575378 |
| Synonyms: |
SM22a-humansPLA2IIA WTB, Tg(Tagln-PLA2G2A)B |
| Transgene: |
Tg(Tagln-PLA2G2A)BMrj Location: unknown
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| Alliance: |
Tg(Tagln-PLA2G2A)BMrj page
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| Strain of Origin: |
C57BL/6 or DBA/2
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(Tagln-PLA2G2A)BMrj expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Homolog in Mouse |
Note |
| human |
PLA2G2A (5320) |
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Mutation details: The 7.2 kb transgene construct contains the promoter region of the mouse transgelin gene (Tgln; also called Sm22a) extending from -2126 to +4136 bp, including 2126 bp of 5' flanking sequence, the first exon, the first intron and the first 11 bp of the second intron, joined to a cDNA encoding human phospholipase A2, group IIA (platelets, synovial fluid) (PLA2G2A) with a C-terminal V5 epitope tag and polyhistidine (6xHis) sequence. Wild-type line B is estimated by Southern blot analysis to carry more than 50 copies of the transgene. Analysis of liver, lung, skeletal muscle, heart and aorta of transgenic mice for transgene-derived PLA2G2A mRNA by reverse transcription- (RT-) PCR analysis and for the protein by western blot analysis demonstrated expression specifically in tissues enriched in smooth muscle, i.e., aorta, heart and lung.
(J:34770, J:209787)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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The transgene construct was "injected into the pronuclei of B6D2 fertilized oocytes." ( J:82809) |
| Original: |
J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013; |
| All: |
3 reference(s) |
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Analysis Tools
