Gene Expression Data
Assay Details
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Assay
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Reference:
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J:166517
Chen G, et al., GPR56 is essential for testis development and male fertility in mice. Dev Dyn. 2010 Dec;239(12):3358-67
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5573250
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Gene symbol:
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Sox9
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Gene name:
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SRY (sex determining region Y)-box 9
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Antibody:
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anti-SOX9
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
3B SOX9 +/-:
postnatal week 10; Adgrg1tm1Lex/Adgrg1 +
(more  )
Specimen
3B SOX9 +/-:
(close  )
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Genetic Background:
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B6.129S5-Adgrg1tm1Lex
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Mutant Allele(s):
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Adgrg1tm1Lex/Adgrg1+
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Age:
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postnatal week 10
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: Sertoli cell |
Sertoli cell |
Present |
Regionally restricted |
3B SOX9 +/-
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Forming tubular structures.
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Specimen
3B SOX9 -/-:
(close )
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Genetic Background:
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B6.129S5-Adgrg1tm1Lex
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Mutant Allele(s):
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Adgrg1tm1Lex/Adgrg1tm1Lex
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Age:
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postnatal week 10
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: Sertoli cell |
Sertoli cell |
Present |
Scattered |
3B SOX9 -/-
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Disorganized distribution was seen in the disrupted seminiferous tubule areas.
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Specimen
S2 E13.5 +/-:
embryonic day 13.5; Adgrg1tm1Lex/Adgrg1 +
(more )
Specimen
S2 E13.5 +/-:
(close )
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Genetic Background:
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B6.129S5-Adgrg1tm1Lex
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Mutant Allele(s):
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Adgrg1tm1Lex/Adgrg1+
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Age:
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embryonic day 13.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
S2 E13.5 -/-:
(close )
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Genetic Background:
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B6.129S5-Adgrg1tm1Lex
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Mutant Allele(s):
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Adgrg1tm1Lex/Adgrg1tm1Lex
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Age:
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embryonic day 13.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: Sertoli cell |
Sertoli cell |
Present |
Regionally restricted |
S2_E13.5 -/-
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Forming tubular structure comparable to the control.
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Specimen
S2 E16.5 +/-:
embryonic day 16.5; Adgrg1tm1Lex/Adgrg1 +
(more )
Specimen
S2 E16.5 +/-:
(close )
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Genetic Background:
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B6.129S5-Adgrg1tm1Lex
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Mutant Allele(s):
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Adgrg1tm1Lex/Adgrg1+
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Age:
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embryonic day 16.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
S2 E16.5 -/-:
(close )
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Genetic Background:
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B6.129S5-Adgrg1tm1Lex
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Mutant Allele(s):
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Adgrg1tm1Lex/Adgrg1tm1Lex
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Age:
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embryonic day 16.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: Sertoli cell |
Sertoli cell |
Present |
Regionally restricted |
S2_E16.5 -/-
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Expressed in testis cords, with disruption of the testis cords on the mesonephric side.
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Analysis Tools