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Gene Expression Data
Assay Details
Assay
Reference: J:166517 Chen G, et al., GPR56 is essential for testis development and male fertility in mice. Dev Dyn. 2010 Dec;239(12):3358-67
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5573247
Gene symbol: Amh
Gene name: anti-Mullerian hormone
Antibody: anti-AMH/MIS (C-20; sc-6886)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1B AMH +/+: embryonic day 14.5 (more )
Note: Double labeled: green - Amh; red - Adgrg1 (Gpr56; assay MGI:5573246).
Structure Cell Type Level Pattern Image Note
TS22: Sertoli cell Sertoli cell Present 1B +/+ Merge , 1B +/+ AMH

Specimen 1B AMH -/-: embryonic day 14.5; Adgrg1tm1Lex/Adgrg1tm1Lex (more )
Note: Double labeled: green - Amh; red - Adgrg1 (Gpr56; assay MGI:5573246).
Structure Cell Type Level Pattern Image Note
TS22: Sertoli cell Sertoli cell Present 1B -/- Merge , 1B -/- AMH

Specimen 4 E12.5 +/-: embryonic day 12.5; Adgrg1tm1Lex/Adgrg1+ (more )
Structure Cell Type Level Pattern Image Note
TS20: Sertoli cell Sertoli cell Present Regionally restricted 4_E12.5 +/- Expressed in developing testis cords.

Specimen 4 E12.5 -/-: embryonic day 12.5; Adgrg1tm1Lex/Adgrg1tm1Lex (more )
Structure Cell Type Level Pattern Image Note
TS20: Sertoli cell Sertoli cell Present Regionally restricted 4_E12.5 -/- Expressed in developing testis cords, comparable to the controls.

Specimen 4 E13.5 +/-: embryonic day 13.5; Adgrg1tm1Lex/Adgrg1+ (more )
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present Regionally restricted 4_E13.5 +/- Expressed in developing testis cords.

Specimen 4 E13.5 -/-: embryonic day 13.5; Adgrg1tm1Lex/Adgrg1tm1Lex (more )
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present Regionally restricted 4_E13.5 -/- Expressed in developing testis cords, comparable to the controls.

Specimen 4 E14.5 +/-: embryonic day 14.5; Adgrg1tm1Lex/Adgrg1+ (more )
Structure Cell Type Level Pattern Image Note
TS22: Sertoli cell Sertoli cell Present Regionally restricted 4_E14.5 +/- Expressed in testis cords.

Specimen 4 E14.5 -/-: embryonic day 14.5; Adgrg1tm1Lex/Adgrg1tm1Lex (more )
Structure Cell Type Level Pattern Image Note
TS22: Sertoli cell Sertoli cell Present Regionally restricted 4_E14.5 -/- Expressed in testis cords, with disruption of the testis cords on the mesonephric side.

Specimen 4 E16.5 +/-: embryonic day 16.5; Adgrg1tm1Lex/Adgrg1+ (more )
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present Regionally restricted 4_E16.5 +/- Expressed in testis cords.

Specimen 4 E16.5 -/-: embryonic day 16.5; Adgrg1tm1Lex/Adgrg1tm1Lex (more )
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present Regionally restricted 4_E16.5 -/- Expressed in testis cords, with disruption of the testis cords on the mesonephric side.

Specimen 4 E18.5 +/-: embryonic day 18.5; Adgrg1tm1Lex/Adgrg1+ (more )
Structure Cell Type Level Pattern Image Note
TS26: Sertoli cell Sertoli cell Present Regionally restricted 4_E18.5 +/- Expressed in testis cords.

Specimen 4 E18.5 -/-: embryonic day 18.5; Adgrg1tm1Lex/Adgrg1tm1Lex (more )
Structure Cell Type Level Pattern Image Note
TS26: Sertoli cell Sertoli cell Present Regionally restricted 4_E18.5 -/- Expressed in testis cords, with disruption of the testis cords on the mesonephric side.

Specimen 5B AMH: embryonic day 16.5; Adgrg1tm1Lex/Adgrg1tm1Lex (more )
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present 5B AMH Expressed in Sertoli cells, which remain attached to laminin.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory