Nkx6-2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:161205 Moreno-Bravo JA, et al., Dynamic expression patterns of Nkx6.1 and Nkx6.2 in the developing mes-diencephalic basal plate. Dev Dyn. 2010 Jul;239(7):2094-101
Assay type:	RNA in situ
MGI Accession ID:	MGI:5572946
Gene symbol:	Nkx6-2
Gene name:	NK6 homeobox 2

Probe:	Nkx6-2 probe1
Probe preparation:	Antisense RNA

Results

1A ©

Specimen 1A: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: future hindbrain floor plate	Present	Regionally restricted	1A	Expression is as a longitudinal stripe.
TS15: future midbrain	Present	Regionally restricted	1A	Expression is as a longitudinal stripe.

1B ©

Specimen 1B: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present		1B
TS17: midbrain	Present		1B

1C ©

Specimen 1C: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present		1C
TS17: forebrain	Present	Regionally restricted	1C	Expression is in the area of the optic stalk.

1D ©

Specimen 1D: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted	1D

1I ©

Specimen 1I: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present		1I

1J ©

Specimen 1J: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted	1J	Expression is in the alar plate.
TS17: midbrain mantle layer	Present	Single cells	1J	Expression is in scattered cells in the basal plate.

1K ©

Specimen 1K: embryonic day 9.5 (more

)
Note: Color-coded overlays of adjacent sections. Colors: green - Nkx6-2; red - Nkx6-1 (assay MGI:5573141).

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	1K	Expression is in the alar plate.

1O ©

Specimen 1O: embryonic day 9.5 (more

)
Note: Color-coded overlays of adjacent sections. Colors: green - Nkx6-2; red - Nkx6-1 (assay MGI:5573141).

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	1O	Expression is in the alar plate.

1L ©

Specimen 1L: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	1L	Expression is in an alar and in a basal domain. The alar domain elongates parallel to the isthmus.
TS19: diencephalon	Present	Regionally restricted	1L	Expression is in the basal area.

2A ©

Specimen 2A: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: midbrain	Strong	Regionally restricted	2A	Expression is a dense population in the basal midbrain.

2D ©

Specimen 2D: embryonic day 11.5 (more

)
Note: Color-coded overlays of adjacent sections. Colors: green - Nkx6-2; red - Nkx6-1 (assay MGI:5573141); blue - Pou4f1 (assay MGI:5573142).

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	2D	Expression is in the basal plate.

2E ©

Specimen 2E: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	2E	Expression is as a longitudinal band along the midbrain and diencephalon close to the ventricular surface in the basal plate.
TS19: midbrain	Present	Regionally restricted	2E	Expression is as a longitudinal band along the midbrain and diencephalon close to the ventricular surface in the basal plate.

2H ©

Specimen 2H: embryonic day 11.5 (more

)
Note: Color-coded overlays of adjacent sections. Colors: green - Nkx6-2; red - Nkx6-1 (assay MGI:5573141); blue - Pou4f1 (assay MGI:5573142).

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	2H	Expression is as a longitudinal band along the midbrain and diencephalon close to the ventricular surface in the basal plate.
TS19: midbrain	Present	Regionally restricted	2H	Expression is as a longitudinal band along the midbrain and diencephalon close to the ventricular surface in the basal plate.

3A ©

Specimen 3A: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	3A	Expression is as a longitudinal band.

3D ©

Specimen 3D: embryonic day 12.5 (more

)
Note: Color-coded overlays of adjacent sections. Colors: green - Nkx6-2; red - Nkx6-1 (assay MGI:5573141); blue - Pou4f1 (assay MGI:5573142).

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	3D	Expression is as a longitudinal band.

3E ©

Specimen 3E: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	3E	Expression is as a longitudinal band.
TS20: midbrain	Present	Regionally restricted	3E	Expression is as a longitudinal band.

3H ©

Specimen 3H: embryonic day 12.5 (more

)
Note: Color-coded overlays of adjacent sections. Colors: green - Nkx6-2; red - Nkx6-1 (assay MGI:5573141); blue - Pou4f1 (assay MGI:5573142).

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	3H	Expression is as a longitudinal band.
TS20: midbrain	Present	Regionally restricted	3H	Expression is as a longitudinal band.

4A ©

Specimen 4A: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: diencephalon	Present	Regionally restricted	4A	Expression is in a few scattered cells in the rostral diencephalon.

4B ©

Specimen 4B: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	4B	Expression is as a longitudinal domain.

4C ©

Specimen 4C: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	4C	Expression is as a longitudinal domain and weak in the trigeminal nucleus and the reticular formation associated with the facial nucleus.

4D ©

Specimen 4D: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	4D	Expression is as a longitudinal domain in the basal plate and is almost absent in the caudal partand is weak in the reticular formation associated with the facial nucleus. In the caudal midbrain expression is in an alar domain.