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Gene Expression Data
Assay Details
Assay
Reference: J:1957 Begley CG, et al., Molecular characterization of NSCL, a gene encoding a helix-loop-helix protein expressed in the developing nervous system. Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):38-42
Assay type: RNA in situ
MGI Accession ID: MGI:5569546
Gene symbol: Nhlh1
Gene name: nescient helix loop helix 1
Probe: Nscl cDNA2
Probe preparation: Antisense labelled with S35 RNA
Visualized with: Autoradiography
Assay notes: Two probes were described, but the one used in this assay was not detailed. Both probes gave identical results. MGI:5569544 and MGI:5569545
Results
Specimen 5: embryonic day 13.0 (more )
Note: C/D and E/F are higher magnifications of the boxed areas in A.
Structure Level Pattern Image Note
TS21: medulla oblongata Present 5A/B , 5C/D Expression is in a narrow strip of cells in the subependymal layer of the neuroepithelium, absent in the mitotically active germinal layer adjacent to the ventricle or in the outer layers.
TS21: spinal cord Present 5E/F , 5A/B Expression is in a narrow strip of cells in the subependymal layer of the neuroepithelium, absent in the mitotically active germinal layer adjacent to the ventricle or in the outer layers.
Specimen 6A/B: embryonic day 13.0 (more )
Structure Level Pattern Image Note
TS21: dorsal root ganglion Strong 6A/B
Specimen 6C/D: embryonic day 13.0 (more )
Structure Level Pattern Image Note
TS21: trigeminal ganglion Strong 6C/D
Specimen 6E/F: embryonic day 13.0 (more )
Structure Level Pattern Image Note
TS21: olfactory epithelium Strong 6E/F
Specimen not shown E12: embryonic day 12.0 (more )
Structure Level Pattern Image Note
TS20: brain Strong
TS20: spinal cord Strong
Specimen not shown E13: embryonic day 13.0 (more )
Structure Level Pattern Image Note
TS21: cranial ganglion Strong
TS21: eye Present Regionally restricted Expression is in the sensory layer.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory