Gene Expression Data
Assay Details
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Assay
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Reference:
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J:146224
Staverosky JA, et al., Tubulin polymerization-promoting protein family member 3, Tppp3, is a specific marker of the differentiating tendon sheath and synovial joints. Dev Dyn. 2009 Mar;238(3):685-92
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5569409
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Gene symbol:
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Tppp3
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Gene name:
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tubulin polymerization-promoting protein family member 3
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Probe:
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MGC clone 18840
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1A:
embryonic day 14.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
|
Not Specified
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Age:
|
embryonic day 14.5
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Sex:
|
Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: forelimb |
Present |
Regionally restricted |
1A
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Expression is in the external outline of tendons, absent in the core. Shown is the Profundus flexor tendon.
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Specimen
1C:
embryonic day 16.5
(more )
Note: F is a higher magnification of the boxed area in C. Double labeled: purple - Tppp3; red - Col2a1 (assay MGI:5569490).
Specimen
1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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F is a higher magnification of the boxed area in C. Double labeled: purple - Tppp3; red - Col2a1 (assay MGI:5569490).
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Specimen
1D:
embryonic day 14.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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Not Specified
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Age:
|
embryonic day 14.5
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: forelimb |
Present |
Regionally restricted |
1D
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Expression is in the external outline of tendons, absent in the core. Shown is the Extensor Policis tendon.
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Specimen
1G:
embryonic day 16.5
(more )
Specimen
1G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hand |
Present |
Regionally restricted |
1G
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Expression is in flexor tendons, absent in extensor tendons.
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Specimen
1I:
embryonic day 14.5
(more )
Specimen
1I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: wrist |
Present |
Regionally restricted |
1I
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Expression surrounds each extensor and flexor tendon in the wrist.
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Specimen
1L:
embryonic day 16.5
(more )
Note: Double labeled: purple - Tppp3; red - Col2a1 (assay MGI:5569490).
Specimen
1L:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: purple - Tppp3; red - Col2a1 (assay MGI:5569490).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: tail |
Present |
Regionally restricted |
1L
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Expression is surrounding tendons of the tail.
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: limb |
Absent |
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Absent in tendon progenitors.
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Specimen
2A:
embryonic day 13.5
(more )
Specimen
2A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forelimb musculature |
Present |
Regionally restricted |
2A
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Expression is in skeletal muscle of the handplate.
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| TS21: hand |
Present |
Regionally restricted |
2A
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Absent in tendon sheaths at the level of the digits or metacarpals, present in skeletal muscle and in a stripe between metacarpals and the forming extensor tendons.
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Specimen
2C:
embryonic day 13.5
(more )
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forelimb musculature |
Present |
Regionally restricted |
2C
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ExprExpression is in skeletal muscle of the handplate.ession is in skeletal muscle of the handplate.
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| TS21: hand |
Present |
Regionally restricted |
2C
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Expression is in the forming tendon sheath of the extensor tendons and the Profundus flexor tendons.
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Specimen
2D:
embryonic day 13.5
(more )
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forelimb musculature |
Present |
Regionally restricted |
2D
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Expression is in skeletal muscle of the handplate.
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| TS21: hand |
Present |
Regionally restricted |
2D
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Expression is in the forming tendon sheath of the extensor tendons and the Profundus flexor tendons.
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Specimen
2E:
embryonic day 14.5
(more )
Specimen
2E:
(close )
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Genetic Background:
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Not Specified
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Age:
|
embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: forelimb musculature |
Present |
Regionally restricted |
2E
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Expression is in skeletal muscle of the handplate.
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| TS22: hand |
Present |
Regionally restricted |
2E
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Expression is in the tendon sheaths, absent in sheats surrounding flexor tendons.
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Specimen
2F:
embryonic day 14.5
(more )
Specimen
2F:
(close )
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Genetic Background:
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Not Specified
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Age:
|
embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: forelimb musculature |
Present |
Regionally restricted |
2F
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Expression is in skeletal muscle of the handplate.
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| TS22: hand |
Present |
Regionally restricted |
2F
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Expression is in the tendon sheaths, absent in sheats surrounding flexor tendons.
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Specimen
2G:
embryonic day 14.5
(more )
Specimen
2G:
(close )
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Genetic Background:
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Not Specified
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Age:
|
embryonic day 14.5
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Sex:
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Not Specified
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Type:
|
section
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|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: forelimb musculature |
Present |
Regionally restricted |
2G
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Expression is in skeletal muscle of the handplate.
|
| TS22: hand |
Present |
Regionally restricted |
2G
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Expression is in the tendon sheaths.
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Specimen
2H:
embryonic day 14.5
(more )
Specimen
2H:
(close )
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Genetic Background:
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Not Specified
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Age:
|
embryonic day 14.5
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|
Sex:
|
Not Specified
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Type:
|
section
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|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hand |
Present |
Regionally restricted |
2H
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Expression is in the tendon sheaths.
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Specimen
2I:
embryonic day 15.5
(more )
Specimen
2I:
(close )
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Genetic Background:
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Not Specified
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Age:
|
embryonic day 15.5
|
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hand |
Present |
Regionally restricted |
2I
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Expression is in the tendon sheaths and is strong at the metacarpal-phalangeal joint.
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Specimen
2J:
embryonic day 15.5
(more )
Specimen
2J:
(close )
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Genetic Background:
|
Not Specified
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Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hand |
Present |
Regionally restricted |
2J
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Expression is in the tendon sheaths.
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Specimen
2K:
embryonic day 15.5
(more )
Specimen
2K:
(close )
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Genetic Background:
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Not Specified
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Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
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Type:
|
section
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|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hand |
Present |
Regionally restricted |
2K
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Expression is in the tendon sheaths and is strong between carpal bones.
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Specimen
2L:
embryonic day 15.5
(more )
Note: Double labeled: purple - Tppp3; red - Col2a1 (assay MGI:5569490).
Specimen
2L:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: purple - Tppp3; red - Col2a1 (assay MGI:5569490).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hand |
Present |
Regionally restricted |
2L
|
Expression is in the tendon sheaths.
|
Specimen
3A:
embryonic day 18.5
(more )
Note: E is a higher magnification of the boxed area in A.
Specimen
3A:
(close )
|
Genetic Background:
|
Not Specified
|
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Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
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Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
E is a higher magnification of the boxed area in A.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hand |
Present |
Regionally restricted |
3E
,
3A
|
Expression is in a thin layer surrounding the tendons, absent in extensor tendons in the digits and distal metacarpals.
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Specimen
3B:
embryonic day 18.5
(more )
Note: G is a higher magnification of the boxed area in B.
Specimen
3B:
(close )
|
Genetic Background:
|
Not Specified
|
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Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
G is a higher magnification of the boxed area in B.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hand |
Present |
Regionally restricted |
3G
,
3B
|
Expression is in a thin layer surrounding the tendons absent in extensor tendons in the digits and distal metacarpals.
|
Specimen
3C:
embryonic day 18.5
(more )
Note: H is a higher magnification of the boxed area in C.
Specimen
3C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
H is a higher magnification of the boxed area in C.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hand |
Present |
Regionally restricted |
3H
,
3C
|
Expression is in a thin layer surrounding the tendons and in skeletal muscle.
|
| TS26: forearm muscle |
Present |
Regionally restricted |
3C
,
3H
|
Expression is in skeletal muscle of the handplate.
|
Specimen
3D:
embryonic day 18.5
(more )
Note: F is a higher magnification of the boxed area in D.
Specimen
3D:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
F is a higher magnification of the boxed area in D.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: forearm |
Present |
|
3D
,
3F
|
Expression is in a thin layer surrounding the tendons in the wrist.
|
Specimen
4C:
embryonic day 14.5
(more )
Specimen
4C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
4D:
embryonic day 14.5
(more )
Specimen
4D:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: wrist |
Present |
Regionally restricted |
4D
|
Expression surrounding tendons, absent in the wrist joint.
|
| TS22: forelimb digit |
Absent |
|
4D
|
Absent in the joints.
|
Specimen
4G:
embryonic day 16.5
(more )
Specimen
4G:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
4H:
embryonic day 16.5
(more )
Specimen
4H:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: forelimb joint |
Present |
Regionally restricted |
4H
|
Expression is in the joints of the digits and wrist.
|
| TS24: hand |
Present |
Regionally restricted |
4H
|
Expression is in the joints of the digits and wrist.
|
Specimen
4I:
embryonic day 15.5
(more )
Specimen
4I:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: forelimb digit |
Strong |
Regionally restricted |
4I
|
Expression is at the metacarpal-phalangeal joint.
|
Specimen
4J:
embryonic day 17.5
(more )
Specimen
4J:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: forelimb digit |
Present |
Regionally restricted |
4J
|
Expression is in dispersed cells at the metacarpal-phalangeal joint.
|
Specimen
4K:
embryonic day 18.5
(more )
Specimen
4K:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: forelimb digit |
Present |
Single cells |
4K
|
Expression is in a few dispersed cells at the metacarpal-phalangeal joint.
|
|
Analysis Tools