Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:140657
Villasenor A, et al., Biphasic Ngn3 expression in the developing pancreas. Dev Dyn. 2008 Nov;237(11):3270-9
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:5565285
|
Gene symbol:
|
Neurog3
|
Gene name:
|
neurogenin 3
|
Probe:
|
Ngn3 cDNA5
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Results
|
Specimen
1A:
embryonic day 8.0
(more )
Note: 5 somites.
Specimen
1A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
5 somites.
|
Specimen
1A inset:
embryonic day 8.0
(more )
Note: 5 somites.
Specimen
1A inset:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
5 somites.
|
Specimen
1B:
embryonic day 8.25
(more )
Note: 9 somites.
Specimen
1B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
9 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: foregut-midgut junction |
Present |
Single cells |
1B
|
Expression is in a few cells within the dorsal pre-pancreatic endoderm.
|
Specimen
1B inset:
embryonic day 8.25
(more )
Note: 9 somites.
Specimen
1B inset:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
9 somites.
|
Specimen
1C:
embryonic day 8.5
(more )
Note: 12 somites.
Specimen
1C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
12 somites.
|
Specimen
1C inset:
embryonic day 8.5
(more )
Note: 12 somites.
Specimen
1C inset:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
12 somites.
|
Specimen
1D:
embryonic day 9.0
(more )
Specimen
1D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1E:
embryonic day 9.5
(more )
Specimen
1E:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2A:
embryonic day 10.0
(more )
Note: Dissected gut tube.
Specimen
2A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Dissected gut tube.
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: pancreas primordium |
Strong |
Regionally restricted |
2A
|
Expression is in the dorsal pancreatic epithelium, faint in the ventral pancreas.
|
Specimen
2B:
embryonic day 10.25
(more )
Note: Dissected gut tube.
Specimen
2B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Dissected gut tube.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: pancreas primordium |
Strong |
Regionally restricted |
2B
|
Expression is in the dorsal pancreatic epithelium.
|
Specimen
2C:
embryonic day 10.5
(more )
Note: Dissected gut tube.
Specimen
2C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Dissected gut tube.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: pancreas primordium |
Strong |
Regionally restricted |
2C
|
Expression is in the dorsal pancreatic epithelium.
|
Specimen
2D:
embryonic day 10.75
(more )
Note: Dissected gut tube.
Specimen
2D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.75
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Dissected gut tube.
|
Specimen
2E:
embryonic day 11.0
(more )
Note: Dissected gut tube.
Specimen
2E:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Dissected gut tube.
|
Specimen
2F:
embryonic day 11.25
(more )
Note: 41 somites.
Specimen
2F:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
41 somites.
|
Specimen
2G:
embryonic day 11.5
(more )
Note: 44 somites.
Specimen
2G:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
44 somites.
|
Specimen
2H:
embryonic day 11.75
(more )
Note: 46 somites.
Specimen
2H:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.75
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
46 somites.
|
Specimen
2I:
embryonic day 12.0
(more )
Specimen
2I:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2J:
embryonic day 12.25
(more )
Specimen
2J:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2K:
embryonic day 12.5
(more )
Specimen
2K:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2L:
embryonic day 13.0
(more )
Specimen
2L:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: pancreas |
Strong |
Regionally restricted |
2L
|
Expression is in the branching epithelium.
|
Specimen
2M:
embryonic day 13.5
(more )
Specimen
2M:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: pancreas |
Present |
Regionally restricted |
2M
|
Expression is in the branching epithelium.
|
Specimen
2N:
embryonic day 14.0
(more )
Specimen
2N:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: pancreas |
Present |
Regionally restricted |
2N
|
Expression is in the branching epithelium.
|
Specimen
2O:
embryonic day 14.5
(more )
Specimen
2O:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: pancreas |
Present |
Regionally restricted |
2O
|
Expression is in the branching epithelium.
|
Specimen
3A:
embryonic day 8.5
(more )
Specimen
3A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3B:
embryonic day 10.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3C:
embryonic day 11.0
(more )
Specimen
3C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3D:
embryonic day 11.25
(more )
Specimen
3D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3E:
embryonic day 12.0
(more )
Specimen
3E:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3F:
embryonic day 12.5
(more )
Specimen
3F:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: pancreas |
Present |
|
3F
|
Higher expression in scattered cells and lower levels throughout the epithelium.
|
Specimen
5B:
embryonic day 13.0
(more )
Specimen
5B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: pancreas |
Present |
Non-Uniform |
5B
|
There are high and low expressing cells thoughout the epithelium.
|
Specimen
S1A:
embryonic day 8.25
(more )
Note: 8 somites.
Specimen
S1A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
8 somites.
|
Specimen
S1B:
embryonic day 8.25
(more )
Note: 9 somites.
Specimen
S1B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
9 somites.
|
Specimen
S1C:
embryonic day 8.25
(more )
Note: 10 somites.
Specimen
S1C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
10 somites.
|
Specimen
S1D:
embryonic day 8.25
(more )
Note: 8 somites.
Specimen
S1D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
8 somites.
|
Specimen
S1E:
embryonic day 8.25
(more )
Note: 9 somites.
Specimen
S1E:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
9 somites.
|
Specimen
S1F:
embryonic day 8.25
(more )
Note: 10 somites.
Specimen
S1F:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
10 somites.
|
Specimen
S2A:
embryonic day 9.5
(more )
Specimen
S2A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: telencephalon |
Present |
Regionally restricted |
S2A
|
Expression is in a small region near Rathke's pouch.
|
Specimen
S2B:
embryonic day 9.5
(more )
Specimen
S2B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: telencephalon |
Present |
Regionally restricted |
S2B
|
Expression is in two patches flanking the midline.
|
Specimen
S2C:
embryonic day 9.5
(more )
Specimen
S2C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
S2D:
embryonic day 10.5
(more )
Specimen
S2D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: telencephalon |
Weak |
Regionally restricted |
S2D
|
Expression is in a small region near Rathke's pouch.
|
Specimen
S2E:
embryonic day 10.5
(more )
Specimen
S2E:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: telencephalon |
Weak |
Regionally restricted |
S2E
|
Expression is in two patches flanking the midline.
|
Specimen
S2F:
embryonic day 10.5
(more )
Specimen
S2F:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
|