Gene Expression Data
Assay Details
Assay
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Reference:
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J:121116
Applebury ML, et al., Transient expression of thyroid hormone nuclear receptor TRbeta2 sets S opsin patterning during cone photoreceptor genesis. Dev Dyn. 2007 May;236(5):1203-12
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5565252
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Gene symbol:
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Thrb
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Gene name:
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thyroid hormone receptor beta
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Probe:
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TRbeta2 cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 15.0
(more )
Specimen
2A:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 15.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS23: retina |
Present |
Regionally restricted |
2A
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Expression is in the outer neuroblast layer.
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Specimen
2B:
embryonic day 15.0
(more )
Specimen
2B:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: retina |
Present |
Regionally restricted |
2B
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Expression is in the outer neuroblast layer.
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Specimen
2D:
postnatal day 60
(more )
Specimen
2D:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 60
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: photoreceptor layer |
Weak |
Regionally restricted |
2D
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Expression is in about 3% of the cells of the outer nuclear layer whose cell bodies lie at the ventricular side of the layer.
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Specimen
2E:
postnatal day 60
(more )
Specimen
2E:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 60
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Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: photoreceptor layer |
Present |
Single cells |
2E
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Expression is in about 3% of the cells of the outer nuclear layer whose cell bodies lie at the ventricular side of the layer.
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Specimen
3A:
embryonic day 15.0
(more )
Specimen
3A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: retina |
Present |
Regionally restricted |
3A
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Uniform across the retinal field but absent in the far periphery.
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Specimen
3B:
postnatal day 1
(more )
Specimen
3B:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS27: retina |
Weak |
Regionally restricted |
3B
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Expression is near the ciliary junctions in both dorsal and ventral areas and is diminished across the central retina.
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Specimen
3C:
postnatal day 5
(more )
Specimen
3C:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina |
Trace |
Regionally restricted |
3C
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Reduced to background levels.
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