Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Csk*)2Weis
Name:	transgene insertion 2, Arthur Weiss
MGI ID:	MGI:5563915
Synonyms:	CskAS
Transgene:	Tg(Csk*)2Weis  Location: unknown
Alliance:	Tg(Csk*)2Weis page

Strain of Origin:

C57BL/6

Transgene Type:    Transgenic (Inserted expressed sequence) Mutation:    Insertion   Tg(Csk*)2Weis expresses 1 gene Transgene expresses: Organism Expressed Gene Note mouse Csk (MGI:88537)   Mutation details: A bacterial artificial chromosome (BAC) library from CHORI was used to obtain a BAC (#RP24-400B5) containing the entire mouse Csk (c-src tyrosine kinase) gene. A point mutation was introduced in Csk at position 266 resulting in a threonine to glycine mutation, inducing a larger ATP-binding pocket to be formed in the expressed protein. To inactivate other genes present in the BAC, both ends of the BAC were deleted, leaving 74kb of sequence, and an in-frame stop codon was inserted in exon 2 of Lman1l (lectin, mannose-binding 1 like) gene. (J:208674)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:208674 Tan YX, et al., Inhibition of the kinase Csk in thymocytes reveals a requirement for actin remodeling in the initiation of full TCR signaling. Nat Immunol. 2014 Feb;15(2):186-94
All:	8 reference(s)