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Gene Expression Data
Assay Details
Assay
Reference: J:143212 Westerlund J, et al., Expression of Islet1 in thyroid development related to budding, migration, and fusion of primordia. Dev Dyn. 2008 Dec;237(12):3820-9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5563383
Gene symbol: Nkx2-1
Gene name: NK2 homeobox 1
Antibody: anti-Nkx2.1 (rabbit)
Detection system: Secondary antibody coupled to rhodamine
Results
Specimen 1A: embryonic day 9.5 (more )
Note: Double labeled: red - Nkx2-1; green - Cdh1 (assay MGI:5563416).
Structure Level Pattern Image Note
TS15: branchial arch endoderm Present 1A Expression is in thyroid progenitor cells in the endoderm lining of the pharyngeal floor.
TS15: thyroid primordium Present 1A Expression is in thyroid progenitor cells in the endoderm lining of the pharyngeal floor.
Specimen 1C: embryonic day 10.75 (more )
Note: Double labeled: red - Nkx2-1; green - Cdh1 (assay MGI:5563416).
Structure Level Pattern Image Note
TS18: thyroid gland Present 1C
Specimen 3A: embryonic day 9.5 (more )
Note: Double labeled: red - Nkx2-1; green - Pecam1 (assay MGI:5563508).
Structure Level Pattern Image Note
TS15: branchial arch endoderm Present 3A
TS15: thyroid primordium Present 3A
Specimen 3B: embryonic day 9.5; Isl1tm1Tmj/Isl1tm1Tmj (more )
Note: Double labeled: red - Nkx2-1; green - Pecam1 (assay MGI:5563508).
Structure Level Pattern Image Note
TS15: branchial arch endoderm Weak Regionally restricted 3B Expression is in the anterior pharyngeal endoderm.
TS15: thyroid primordium Weak 3B
Specimen 4B: embryonic day 10.5 (more )
Note: Double labeled: red - Nkx2-1; green - Cdh1 (assay MGI:5563416).
Structure Level Pattern Image Note
TS17: 4th branchial arch Present Single cells 4B Expression is in the ultimobranchial body.
Specimen 4D: embryonic day 10.75 (more )
Note: Double labeled: red - Nkx2-1; green - Cdh1 (assay MGI:5563416).
Structure Level Pattern Image Note
TS18: 4th branchial arch Present 4D Expression is in the ultimobranchial body.
Specimen 5A: embryonic day 12.5 (more )
Note: Double labeled: red - Nkx2-1; green - Cdh1 (assay MGI:5563416).
Structure Level Pattern Image Note
TS20: embryo Present Regionally restricted 5A Expression is in the epithelium of the paired ultimobranchial bodies and the laryngotracheal groove.
Specimen 5C: embryonic day 13.0 (more )
Note: Double labeled: red - Nkx2-1; green - Cdh1 (assay MGI:5563416).
Structure Level Pattern Image Note
TS21: trachea Present 5C
TS21: thyroid gland Present Regionally restricted 5C Expression is in the midline.
TS21: embryo Present Regionally restricted 5C Expression is in the ultimobranchial body.
TS21: esophagus Absent 5C
Specimen 7A: embryonic day 15.5 (more )
Note: Double labeled: red - Nkx2-1; green - Cdh1 (assay MGI:5563416).
Structure Level Pattern Image Note
TS23: thyroid gland lobe Present Regionally restricted 7A Expression is in the peripheral regions.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory