Gene Expression Data
Assay Details
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Assay
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Reference:
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J:142644
Mahtab EA, et al., Podoplanin deficient mice show a rhoa-related hypoplasia of the sinus venosus myocardium including the sinoatrial node. Dev Dyn. 2008 Dec 18;238(1):183-193
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5563342
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Gene symbol:
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Wt1
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Gene name:
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WT1 transcription factor
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Antibody:
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anti-WT1 (C-19; sc-192)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
1a,b:
embryonic day 10.5
(more  )
Note: b is a higher magnification of the boxed area in a.
Specimen
1a,b:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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b is a higher magnification of the boxed area in a.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: heart |
Present |
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1a
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Expression is in the single layer of mesothelium of the coleomic cavity and epicardium.
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| TS17: pericardium |
Present |
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1a
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Expression is in the single layer of mesothelium of the coleomic cavity and epicardium.
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| TS17: pericardial component |
Present |
Regionally restricted |
1b
,
1a
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Expression is in the epithelium of the coelomic cavity and at the corners of the coelomic cavity.
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Specimen
1e,f:
embryonic day 10.5; Pdpntm1Acgg/ Pdpntm1Acgg
(more )
Note: f is a higher magnification of the boxed area in e.
Specimen
1e,f:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss
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Mutant Allele(s):
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Pdpntm1Acgg/Pdpntm1Acgg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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f is a higher magnification of the boxed area in e.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: heart |
Present |
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1f
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1e
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Expression is disorganized in the mesothelium of the coleomic cavity and epicardium in this mutant.
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| TS17: pericardium |
Present |
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1f
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1e
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Expression is disorganized in the mesothelium of the coleomic cavity and epicardium in this mutant.
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| TS17: pericardial component |
Present |
Regionally restricted |
1e
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1f
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Expression is in the epithelium of the coelomic cavity and is disorganized in this mutant.
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Analysis Tools