Cdx2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:142645 Sherwood RI, et al., Transcriptional dynamics of endodermal organ formation. Dev Dyn. 2008 Dec 18;238(1):29-42
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5562893
Gene symbol:	Cdx2
Gene name:	caudal type homeobox 2

Results

Specimen 3A: embryonic day 9.5 (more

)
Note: Boxed region is magnified in right panel. Double labeled: green - Cdx2; red - Foxa2 (assay MGI:5562658).

Structure	Level	Pattern	Image	Note
TS15: embryo endoderm	Present		3A right panel , 3A left panel	Expression is in the intestine area.
TS15: gut	Present		3A right panel , 3A left panel	Expression is in the intestine area.

3C Cdx2 ©

3C far right ©

Specimen 3C: embryonic day 9.5 (more

)
Note: Triple labeled: red - Cdx2; green - Onecut1 (assay MGI:5562907); blue - Sox2 (assay MGI:5562906).

Structure	Level	Pattern	Image	Note
TS15: embryo endoderm	Present	Regionally restricted	3C far right , 3C Cdx2	Expression is in the posterior endoderm.
TS15: gut	Present	Regionally restricted	3C far right , 3C Cdx2	Expression is in the posterior endoderm.

3D Cdx2 ©

3D far right ©

Specimen 3D: embryonic day 9.5 (more

)
Note: Triple labeled: red - Cdx2; green - Pdx1 (assay MGI:5562896); blue - Sox2 (assay MGI:5562906).

Structure	Level	Pattern	Image	Note
TS15: embryo endoderm	Present	Regionally restricted	3D Cdx2 , 3D far right	Expression is in the posterior endoderm.
TS15: gut	Present	Regionally restricted	3D Cdx2 , 3D far right	Expression is in the posterior endoderm.

Specimen not shown: embryonic day 7.75 (more

)

Structure	Level	Pattern	Image	Note
TS12: gut endoderm	Present	Regionally restricted		Expression is in the most anterior and posterior cells.

Specimen 4A: embryonic day 8.25 (more

)
Note: 4-6 somites. Triple labeled: red - Cdx2; green - Sox2 (assay MGI:5562906); blue - Foxa2 (assay MGI:5562658).

Structure	Level	Pattern	Image	Note
TS13: gut endoderm	Present	Regionally restricted	4A left panel , 4A right panel	Expression extends anteriorly at the stomach-intestine border.

4B ©

Specimen 4B: embryonic day 8.25 (more

)
Note: 9-11 somites. Double labeled: red - Cdx2; green - Sox2 (assay MGI:5562906).

Structure	Level	Pattern	Image	Note
TS13: gut	Present	Regionally restricted	4B	Expression extends anteriorly at the stomach-intestine border.

Specimen 4C: embryonic day 8.75 (more

)
Note: 13-14 somites. Triple labeled: red - Cdx2; blue - Pdx1 (assay MGI:5562896); green - Sox2 (assay MGI:5562906).

Structure	Level	Pattern	Image	Note
TS14: gut	Present	Regionally restricted	4C left panel , 4C right panel	Expression extends to the foregut-midgut junction.

Specimen 4H: embryonic day 9.5 (more

)
Note: 8-6 somites. Triple labeled: red - Cdx2; blue - Onecut1 (assay MGI:5562907); green - Sox2 (assay MGI:5562906).

Structure	Level	Pattern	Image	Note
TS15: gut	Present	Regionally restricted	4H right panel

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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