Pdyntm1.1(cre)Mjkr
Targeted Allele Detail
|
Symbol: |
Pdyntm1.1(cre)Mjkr |
Name: |
prodynorphin; targeted mutation 1.1, Michael J Krashes |
MGI ID: |
MGI:5562873 |
Synonyms: |
Dyn-Cre, Pdyn-IRES-Cre, Prodynorphin-cre |
Gene: |
Pdyn Location: Chr2:129528485-129541764 bp, - strand Genetic Position: Chr2, 63.19 cM
|
Alliance: |
Pdyntm1.1(cre)Mjkr page
|
|
Germline Transmission: |
Earliest citation of germline transmission:
J:207472
|
Parent Cell Line: |
W4 (ES Cell)
|
Strain of Origin: |
129S6/SvEvTac
|
|
Allele Type: |
|
Targeted (Recombinase) |
Mutation: |
|
Insertion
|
|
|
Pdyntm1.1(cre)Mjkr expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Pdyn (MGI:97535) |
|
|
|
|
Mutation details: Using recombineering methods, a selection cassette containing an internal ribosomal entry sequence (IRES) linked to cre and an frt-flanked kanamycin gene was inserted immediately downstream of the stop codon in exon 2 of the Pdyn gene in a BAC. This construct was used to isolate a targeting plasmid with the cre-kanamycin cassette with upstream and downstream Pdyn homology arms. ES cells were targeted and used for blastocyst injection. Chimeras were bred for germline transmission. The selection marker was excised by breeding to Flp deleter animals.
(J:207472)
|
|
|
Activity: |
Tissue activity of this recombinase allele
|
Driver:
|
Pdyn
(mouse)
|
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
|
Original: |
J:207472 Krashes MJ, et al., An excitatory paraventricular nucleus to AgRP neuron circuit that drives hunger. Nature. 2014 Mar 13;507(7491):238-42 |
All: |
45 reference(s) |
|