Probe:	GnRH-1 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Assay notes:	Whole embryo and heads were either frozen for sectioning using a cryostat or post-fixed in 4% formaldehyde and embedded in 10% gelatin for cutting on a vibratome.

Structure	Level	Pattern	Image	Note
TS22: inner enamel epithelium	Present		1C/D	Expression is in cells of the outer and inner dental epithelia.
TS22: lower jaw incisor epithelium	Present		1C/D	Expression is in cells of the outer and inner dental epithelia.
TS22: outer enamel epithelium	Present		1C/D	Expression is in cells of the outer and inner dental epithelia.
TS22: upper jaw incisor epithelium	Present		1C/D	Expression is in cells of the outer and inner dental epithelia.
TS22: lower jaw incisor enamel organ	Present		1C/D	Expression is in the stellate reticulum.
TS22: stellate reticulum	Present		1C/D	Expression is in the stellate reticulum.
TS22: upper jaw incisor enamel organ	Present		1C/D	Expression is in the stellate reticulum.
TS22: oral epithelium	Present	Regionally restricted	1C/D	Expression is in cells in the anterior aspect.

Structure	Level	Pattern	Image	Note
TS22: lower jaw incisor	Present		1E , 1E inset
TS22: lower jaw molar	Absent		1E

Structure	Level	Pattern	Image	Note
TS23: lower jaw incisor epithelium	Present	Regionally restricted	1F	Expression is in the inner dental epithelium.
TS23: lower jaw incisor dental papilla	Absent		1F

Structure	Level	Pattern	Image	Note
TS25: upper jaw incisor epithelium	Present	Regionally restricted	1J inset , 1J/K	Expression is in cells of the outer dental epithelium on the labial surface.
TS25: upper jaw incisor enamel organ	Present	Regionally restricted	1J inset , 1J/K	Expression is in preameloblasts.
TS25: upper jaw incisor dental papilla	Absent		1J inset , 1J/K

Structure	Level	Pattern	Image	Note
TS25: upper jaw molar	Absent		1L , 1L inset

Structure	Level	Pattern	Image	Note
TS27: lower jaw incisor	Present	Regionally restricted	2A	Expression is in ameloblasts and papillary layer cells.
TS27: enamel	Absent		2A