Antibody:	anti-pro-LHRH antibody (SW-1)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Whole embryo and heads were either frozen for sectioning using a cryostat or post-fixed in 4% formaldehyde and embedded in 10% gelatin for cutting on a vibratome.

Structure	Level	Pattern	Image	Note
TS22: incisor epithelium	Present			Expression is in cells of the outer and inner dental epithelia.
TS22: inner enamel epithelium	Present			Expression is in cells of the outer and inner dental epithelia.
TS22: outer enamel epithelium	Present			Expression is in cells of the outer and inner dental epithelia.
TS22: incisor enamel organ	Present	Regionally restricted		Expression is in the stellate reticulum.
TS22: stellate reticulum	Present
TS22: oral epithelium	Present	Regionally restricted		Expression is in cells in the anterior aspect.
TS22: molar	Absent

Structure	Level	Pattern	Image	Note
TS23: lower jaw incisor epithelium	Present	Regionally restricted	1H , 1G	Expression is in the inner and outer dental epithelium.
TS23: inner enamel epithelium	Present		1G , 1H	Expression is in the inner and outer dental epithelium.
TS23: outer enamel epithelium	Present		1G , 1H	Expression is in the inner and outer dental epithelium.
TS23: lower jaw incisor enamel organ	Present	Regionally restricted	1H , 1I , 1G	Expression is in the stellate reticular layer and at the junction of the the enamel knot.
TS23: stellate reticulum	Present		1H , 1I , 1G
TS23: lower jaw incisor dental papilla	Absent		1H , 1G

Structure	Level	Pattern	Image	Note
TS27: lower jaw incisor	Present	Regionally restricted	2B	Expression is in ameloblasts and papillary layer cells.
TS27: enamel	Absent		2B

Structure	Level	Pattern	Image	Note
TS28: upper jaw incisor	Present	Graded	2D , 2E , 2D inset , 2G	An increase in expression from proximally-located cells including preameloblasts, early ameloblasts, and outer dental epithelial cells to distally-located (towards the incisal end) cells including secretory ameloblasts and papillary layer cells.
TS28: enamel	Present	Regionally restricted	2D	Expression is present at the level of secretory ameloblasts.

Structure	Level	Pattern	Image	Note
TS28: molar	Absent

Structure	Level	Pattern	Image	Note
TS28: molar	Absent		3A

Structure	Level	Pattern	Image	Note
TS28: incisor	Present	Regionally restricted	3D	Expression is in epithelial-derived structures.
TS28: incisor dental papilla	Absent		3D

Structure	Level	Pattern	Image	Note
TS22: incisor	Present	Regionally restricted	5B

Structure	Level	Pattern	Image	Note
TS25: incisor	Present	Regionally restricted	5D , 5F