Probe:	GoPro49 cDNA1
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS20: pre-cartilage condensation	Present		2A
TS20: axial skeleton	Strong	Regionally restricted	2A	Strong expression was detected in the vertebrae in the trunk. This region showed co-expression with Col2.
TS20: face	Present	Regionally restricted	2A	Expression was seen in the snout. This region showed co-expression with Col2.
TS20: tongue mesenchyme	Present		2A
TS20: tongue epithelium	Absent		2A
TS20: brain	Weak		2A	There was no overlapping expression with Col2 in the brain.

Structure	Level	Pattern	Image	Note
TS20: axial skeleton	Present		3A	Expression was detected in all vertebrae. Expression was seen in areas were Col2 expression was strong.

Structure	Level	Pattern	Image	Note
TS22: axial skeleton	Present	Non-Uniform	3B	Expression was detected more strongly in more posterior vertebrae. Expression was decreasing from E14.5 onward. Expression was seen in areas were Col2 expression was strong.

Structure	Level	Pattern	Image	Note
TS24: axial skeleton	Present	Non-Uniform	3C	Expression was detected more strongly in more posterior vertebrae but was decreased from earlier stages. Expression was seen in areas where Col2 expression was strong.

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		3J
TS21: spinal cord floor plate	Present		3J

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Present		3K

Structure	Level	Pattern	Image	Note
TS24: axial skeleton	Present	Regionally restricted	3L	Expression was limited to mainly columnar, proliferating chondrocytes. Little or no expression was in the hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS20: hindlimb interdigital region	Present		4A	The interdigital tissue had the highest expression.

Structure	Level	Pattern	Image	Note
TS22: metatarsus cartilage condensation	Present	Regionally restricted	4B	Expression was limited to mainly columnar proliferating chondrocytes with some expression in the prehypertrophic chondrocytes, but no signal in the hypertrophic chondrocytes.
TS22: hindlimb interdigital region	Present		4B	The interdigital tissue had the highest expression.

Structure	Level	Pattern	Image	Note
TS24: metatarsus	Present	Regionally restricted	4C	Expression was limited to mainly columnar proliferating chondrocytes with some expression in the prehypertrophic chondrocytes, but no signal in the hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS22: radius cartilage condensation	Present	Regionally restricted	4G	Expression was detected in the diaphysis. Expression was lower compared to that seen in the vertebrae. Expression was seen in the proliferating, columnar chondrocytes.
TS22: ulna cartilage condensation	Present	Regionally restricted	4G	Expression was detected in the diaphysis. Expression was lower compared to that seen in the vertebrae. Expression was seen in the proliferating, columnar chondrocytes.

Structure	Level	Pattern	Image	Note
TS20: brain	Weak		5A
TS20: face	Present	Regionally restricted	5A	Expression was seen in the snout.
TS20: tongue mesenchyme	Present		5A
TS20: tongue epithelium	Absent		5A
TS20: oral region	Present	Regionally restricted	5A	Expression was detected in tissues underlying the tongue.
TS20: Meckel's cartilage	Present		5A
TS20: mandibular process mesenchyme	Present	Regionally restricted	5A	Expression was detected in the mesenchymal condensation of cartilages in maxilla and mandible.
TS20: maxillary process mesenchyme	Present	Regionally restricted	5A	Expression was detected in the mesenchymal condensation of cartilages in maxilla and mandible.

Structure	Level	Pattern	Image	Note
TS28: nasal septum	Present		5B

Structure	Level	Pattern	Image	Note
TS22: nasal septum	Strong		5C
TS22: face mesenchyme	Strong	Regionally restricted	5C	Expression was intense in the cartilages surrounding the nasal cavity.

Structure	Level	Pattern	Image	Note
TS24: nasal septum	Present		5D

Structure	Level	Pattern	Image	Note
TS20: nasal septum	Present	Regionally restricted	5E	Expression was detected in the region of the future nasal septum.

Structure	Level	Pattern	Image	Note
TS20: cranium	Present	Regionally restricted	5F	Expression was detected in the cartilage of the basioccipital bone.

Structure	Level	Pattern	Image	Note
TS20: jaw primordium mesenchyme	Strong	Regionally restricted	6A	Expression was intense in the the dental follicle, with no expression in the dental papilla or other tooth forming tissues.

Structure	Level	Pattern	Image	Note
TS20: jaw primordium mesenchyme	Strong	Regionally restricted	6B	Expression was intense in the the dental follicle, with no expression in the dental papilla or other tooth forming tissues.

Structure	Level	Pattern	Image	Note
TS20: jaw primordium mesenchyme	Strong	Regionally restricted	6C	Expression was intense in the the dental follicle, with no expression in the dental papilla or other tooth forming tissues.

Structure	Level	Pattern	Image	Note
TS22: dental sac	Strong		6D
TS22: dental papilla	Absent		6D

Structure	Level	Pattern	Image	Note
TS22: Meckel's cartilage	Strong		6E
TS22: dental sac	Strong		6E
TS22: dental papilla	Absent		6E

Structure	Level	Pattern	Image	Note
TS22: dental sac	Strong		6F
TS22: dental papilla	Absent		6F

Structure	Level	Pattern	Image	Note
TS24: dental sac	Strong		6G
TS24: dental papilla	Absent		6G

Structure	Level	Pattern	Image	Note
TS24: dental sac	Strong		6H
TS24: dental papilla	Absent		6H

Structure	Level	Pattern	Image	Note
TS24: dental sac	Strong		6I
TS24: dental papilla	Absent		6I

Structure	Level	Pattern	Image	Note
TS27: dental sac	Strong		6J	Expression became more intense when the follicle cells condensed to form a capsule around the tooth.
TS27: incisor	Present		6J
TS27: lower jaw molar	Present		6J
TS27: upper jaw molar	Present		6J
TS27: dental papilla	Absent		6J

Structure	Level	Pattern	Image	Note
TS27: dental sac	Strong		6K	Expression became more intense when the follicle cells condensed to form a capsule around the tooth.
TS27: dental papilla	Absent		6K

Structure	Level	Pattern	Image	Note
TS28: dental sac	Strong		6L	Expression became more intense when the follicle cells condensed to form a capsule around the tooth.
TS28: incisor	Present		6L
TS28: lower jaw molar	Present		6L
TS28: upper jaw molar	Present		6L
TS28: dental papilla	Absent		6L

Structure	Level	Pattern	Image	Note
TS28: dental sac	Strong		6M	Expression became more intense when the follicle cells condensed to form a capsule around the tooth.
TS28: dental papilla	Absent		6M

Structure	Level	Pattern	Image	Note
TS27: vibrissa follicle	Present		6O
TS27: hair follicle	Weak		6O	Lower expression levels were noted in hair follicles in the head region.
TS27: dermis papillary layer	Ambiguous	Regionally restricted	6O	Little or no expression was detected in the dermal papilla in the vibrissa follicles.

Structure	Level	Pattern	Image	Note
TS28: dermis papillary layer	Present		6P	In hair follicles, expression was in the dermal papilla and in the surrounding mesenchyme.

Structure	Level	Pattern	Image	Note
TS28: hair root sheath	Strong	Regionally restricted	6Q	Strongest expression was observed in the multiple layers of the root sheath of the vibrissa follicles.