Esr1tm1.1(cre)And
Targeted Allele Detail
|
Symbol: |
Esr1tm1.1(cre)And |
Name: |
estrogen receptor 1 (alpha); targeted mutation 1.1, David J Anderson |
MGI ID: |
MGI:5559339 |
Synonyms: |
Esr1cre, Esr1cre |
Gene: |
Esr1 Location: Chr10:4561989-4955633 bp, + strand Genetic Position: Chr10, 2.03 cM
|
Alliance: |
Esr1tm1.1(cre)And page
|
|
Germline Transmission: |
Earliest citation of germline transmission:
J:207677
|
Parent Cell Line: |
TC1/TC-1 (ES Cell)
|
Strain of Origin: |
129S6/SvEvTac
|
|
Allele Type: |
|
Targeted (RMCE-ready, Recombinase) |
Mutation: |
|
Insertion
|
|
|
Esr1tm1.1(cre)And expression driven by
1 gene
Knock-in expression driven by:
|
|
|
Mutation details: The Esr1-cre targeting vector was designed to insert an attP site, a 2A oligopeptide (mediates ribosomal skipping), the Cre recombinase gene, a frt-flanked PGKneo-polyA cassette, and an attP site within the 3' UTR of the estrogen receptor 1 alpha gene (Esr1). The targeting construct was electroporated into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. Chimeric males were bred with 129S6/SvEvTac females to establish the colony. Heterozygotes were bred to ACT-FLPe mice to remove the frt-flanked PGKneo-polyA cassette. Mice were bred to remove the ACT-FLPe transgene from the background.
(J:207677)
|
|
|
Activity: |
Tissue activity of this recombinase allele
|
Driver:
|
Esr1
(mouse)
|
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
|
|
Original: |
J:207677 Lee H, et al., Scalable control of mounting and attack by Esr1+ neurons in the ventromedial hypothalamus. Nature. 2014 May 29;509(7502):627-32 |
All: |
28 reference(s) |
|