Probe:	Atx cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: neural fold	Present	Regionally restricted	1A	Expression was detected in a narrow stripe in the cephalic neural folds.

Structure	Level	Pattern	Image	Note
TS13: neural fold	Present	Regionally restricted	1A'	Expression was detected in the anterior neural folds posterior to the future closure of the neural tube at the forebrain-midbrain boundary.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present	Regionally restricted	1B	The stripe of expression was almost overlapping with the posterior limit of Wnt1 expression in the midbrain.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present	Regionally restricted	1C	Expression was detected in the midbrain abutting the midbrain-hindbrain boundary. Expression was anterior to the Gbx2 expression domain of the hindbrain.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present	Regionally restricted	1C"	Expression was detected in the midbrain abutting the midbrain-hindbrain boundary. Expression was anterior to the Gbx2 expression domain of the hindbrain.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present	Regionally restricted	1D	Expression was detected in the midbrain abutting the midbrain-hindbrain boundary. Expression was immediately anterior to the Pax2 expression domain of the midbrain-hindbrain boundary.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present	Regionally restricted	1E	Expression was detected in the midbrain abutting the midbrain-hindbrain boundary. Expression was immediately anterior to the Fgf8 expression domain of the midbrain-hindbrain boundary.

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Weak		1F	Expression at the posterior end of the midbrain and in the vicinity of the midbrain-hindbrain boundary was weak.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch	Present		1H
TS15: branchial arch	Present		1H
TS15: nasal process	Present		1H
TS15: otocyst	Present		1H
TS15: future midbrain	Trace	Regionally restricted	1H	Expression was barely detected near the midbrain-hindbrain boundary.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present		1I
TS15: future midbrain	Trace	Regionally restricted	1I	Expression was barely detected near the midbrain-hindbrain boundary.

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present		1J
TS17: latero-nasal process	Present		1J
TS17: limb	Present		1J
TS17: medial-nasal process	Present		1J
TS17: neural tube	Present		1J
TS17: hindbrain roof plate	Present	Regionally restricted	1J	Expression was detected in the roof of the caudal hindbrain.

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		1J'

Structure	Level	Pattern	Image	Note
TS17: branchial arch mesenchyme	Present	Regionally restricted	1J"	Expression was detected in the subepithelial mesenchymal cells of the branchial arches.

Structure	Level	Pattern	Image	Note
TS17: nasal process	Present		1K

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present		1K'

Structure	Level	Pattern	Image	Note
TS17: hindbrain roof plate	Present		1L

Structure	Level	Pattern	Image	Note
TS17: rhombomere 1	Absent		1M	Expression was not found in the upper rhombic lip region.

Structure	Level	Pattern	Image	Note
TS17: rhombomere roof plate	Present	Regionally restricted	1N	Expression was detected in the roof plate of the lower rhombic lip region.
TS17: hindbrain roof plate	Present	Regionally restricted	1N	Expression was detected in the lower rhombic lip roof plate, but the midline roof plate cells of the hindbrain were devoid of expression.

Structure	Level	Pattern	Image	Note
TS17: rhombomere roof plate	Present	Regionally restricted	1O	Expression was detected in the roof plate of the lower rhombic lip region.
TS17: hindbrain roof plate	Present	Regionally restricted	1O	Expression was detected in the lower rhombic lip roof plate, but the midline roof plate cells of the hindbrain were devoid of expression.

Structure	Level	Pattern	Image	Note
TS19: 1st branchial arch	Present		1P
TS19: hindbrain	Present		1P
TS19: limb	Present		1P
TS19: maxillary process	Present		1P
TS19: nasal process	Present		1P
TS19: neural tube	Present		1P
TS19: branchial arch	Present		1P	Expression was detected in the branchial region.

Structure	Level	Pattern	Image	Note
TS19: 1st branchial arch	Present		1P inset	Expression was detected in the branchial region.
TS19: branchial arch	Present		1P inset	Expression was detected in the branchial region.

Structure	Level	Pattern	Image	Note
TS20: ear	Present		1Q
TS20: limb	Present		1Q
TS20: vibrissa	Present		1Q
TS20: eye	Present	Regionally restricted	1Q	Expression was detected in the eyelid canthus.
TS20: integumental system	Present	Regionally restricted	1Q	Expression was detected in the mammary glands.

Structure	Level	Pattern	Image	Note
TS20: intestine	Present

Structure	Level	Pattern	Image	Note
TS20: choroid plexus	Present		1R
TS20: genital tubercle	Present		1R
TS20: axial skeleton	Present		1R	Expression was detected in the vertebra.

Structure	Level	Pattern	Image	Note
TS21: 4th ventricle choroid plexus	Present		1U , 1S
TS21: medulla oblongata ventricular layer	Present		1U , 1S
TS21: pons ventricular layer	Present		1U , 1S
TS21: embryo mesenchyme	Present	Regionally restricted	1V , 1S , 1W	Expression was detected in the subepithelial mesenchyme of pharyngeal and cervical regions (V) and the subepithelial mesenchyme lining the embryo (W).
TS21: vibrissa dermal component	Present	Regionally restricted	1S , 1X	Expression was detected in the dermal papilla of the vibrissae.

Structure	Level	Pattern	Image	Note
TS21: upper eyelid mesenchyme	Present		1S inset

Structure	Level	Pattern	Image	Note
TS21: 4th ventricle choroid plexus	Present		1T

Structure	Level	Pattern	Image	Note
TS21: cochlear duct mesenchyme	Present		1Y

Structure	Level	Pattern	Image	Note
TS21: thymus primordium	Present		1Y'

Structure	Level	Pattern	Image	Note
TS21: genital tubercle	Present	Regionally restricted	1Z	Expression was detected in the subepithelial mesenchyme of the genital tubercle.

Structure	Level	Pattern	Image	Note
TS21: diaphragm	Present		1Z'