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Gene Expression Data
Assay Details
Assay
Reference: J:122404 Kim KE, et al., Lymphatic development in mouse small intestine. Dev Dyn. 2007 Jul;236(7):2020-5
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5557998
Gene symbol: Lyve1
Gene name: lymphatic vessel endothelial hyaluronan receptor 1
Antibody: anti-LYVE-1
Detection system: Secondary antibody coupled to other - see notes
Assay notes: Fixative for whole mounts was 1% paraformaldehyde. The authors did not specify which Lyve1 antibody was used. Either this one or a rat monoclonal antibody MGI:5556292. The secondary antibody was labeled with either FITC or Cy5.
Results
Specimen 1D: embryonic day 16.5 (more )
Note: Double labeled: red - Lyve1; green - Adgre1 (F4/80; assay MGI:5558003).
Structure Level Pattern Image Note
TS24: intestine Present Regionally restricted 1D Merged , 1D LYVE-1 Expression is in lymphatic vasculatures and in scattered macrophages.
TS24: lymphatic system Present Regionally restricted 1D Merged , 1D LYVE-1 Expression is in lymphatic vasculatures and in scattered macrophages.
Specimen 3C: embryonic day 17.5 (more )
Note: Double labeled: red - Lyve1; green - Prox1 (assay MGI:5558009).
Structure Level Pattern Image Note
TS25: intestine Present Regionally restricted 3C Expression is in lymphatic endothelial cells and macrophages.
TS25: lymphatic system Present Regionally restricted 3C Expression is in lymphatic endothelial cells and macrophages.
Specimen 3D: embryonic day 17.5 (more )
Note: Double labeled: green - Lyve1; red - Pdpn (assay MGI:5558011).
Structure Level Pattern Image Note
TS25: intestine Strong Regionally restricted 3D LYVE-1 , 3D Merged Expression is in the large lymphatic vessels of the mesentery and in most of the branching lymphatics of the intestinal wall.
TS25: lymphatic system Strong Regionally restricted 3D LYVE-1 , 3D Merged Expression is in the large lymphatic vessels of the mesentery and in most of the branching lymphatics of the intestinal wall.
Specimen 3E: embryonic day 17.5 (more )
Note: Double labeled: green - Lyve1; red - Flt4 (VEGFR3; assay MGI:5558012).
Structure Level Pattern Image Note
TS25: intestine Strong Regionally restricted 3E LYVE-1 , 3E Merged Expression is in the branching lymphatics of the intestinal wall and in macrophages.
TS25: lymphatic system Strong Regionally restricted 3E LYVE-1 , 3E Merged Expression is in the branching lymphatics of the intestinal wall and in macrophages.
Specimen 4A E13.5 PECAM-1: embryonic day 13.5 (more )
Note: Double labeled: green - Lyve1; red - Pecam1 (assay MGI:5558013).
Structure Level Pattern Image Note
TS21: intestine Present Single cells 4A PECAM-1/LYVE-1 E13.5 Expression is in a few scattered cells on the surface of the intestine.
Specimen 4A E14.5 PECAM-1: embryonic day 14.5 (more )
Note: Double labeled: green - Lyve1; red - Pecam1 (assay MGI:5558013).
Structure Level Pattern Image Note
TS22: intestine Present Regionally restricted 4A PECAM-1/LYVE-1 E14.5 Expression is in a few scattered cells.
Specimen 4A E15.5 PECAM-1: embryonic day 15.5 (more )
Note: Double labeled: green - Lyve1; red - Pecam1 (assay MGI:5558013).
Structure Level Pattern Image Note
TS23: blood vessel Present Regionally restricted 4A PECAM-1/LYVE-1 E15.5 Expression is in blood vessel and lymphatic endothelial cells.
TS23: intestine Present Regionally restricted 4A PECAM-1/LYVE-1 E15.5 Expression is in blood vessel and lymphatic endothelial cells.
TS23: lymphatic system Present Regionally restricted 4A PECAM-1/LYVE-1 E15.5 Expression is in blood vessel and lymphatic endothelial cells.
Specimen 4A E17.5 PECAM-1: embryonic day 17.5 (more )
Note: Double labeled: green - Lyve1; red - Pecam1 (assay MGI:5558013).
Structure Level Pattern Image Note
TS25: intestine Present Regionally restricted 4A PECAM-1/LYVE-1 E17.5 Expression is in lymphatic endothelial cells.
TS25: lymphatic system Present Regionally restricted 4A PECAM-1/LYVE-1 E17.5 Expression is in lymphatic endothelial cells.
Specimen 4A E13.5 Prox1: embryonic day 13.5 (more )
Note: Double labeled: green - Lyve1; blue - Prox1 (assay MGI:5558009).
Structure Level Pattern Image Note
TS21: intestine Present Single cells 4A LYVE-1/Prox-1 E13.5 Expression is in a few scattered cells on the surface of the intestine.
Specimen 4A E14.5 Prox1: embryonic day 14.5 (more )
Note: Double labeled: green - Lyve1; blue - Prox1 (assay MGI:5558009).
Structure Level Pattern Image Note
TS22: intestine Present Single cells 4A LYVE-1/Prox-1 E14.5 Expression is in a few scattered cells.
Specimen 4A E15.5 Prox1: embryonic day 15.5 (more )
Note: Double labeled: green - Lyve1; blue - Prox1 (assay MGI:5558009).
Structure Level Pattern Image Note
TS23: blood vessel Present Regionally restricted 4A LYVE-1/Prox-1 E15.5 Expression is in blood vessel and lymphatic endothelial cells.
TS23: lymphatic system Present Regionally restricted 4A LYVE-1/Prox-1 E15.5 Expression is in blood vessel and lymphatic endothelial cells.
Specimen 4A E17.5 Prox1: embryonic day 17.5 (more )
Note: Double labeled: green - Lyve1; blue - Prox1 (assay MGI:5558009).
Structure Level Pattern Image Note
TS25: intestine Present Regionally restricted 4A LYVE-1/Prox-1 E17.5 Expression is in lymphatic endothelial cells.
TS25: lymphatic system Present Regionally restricted 4A LYVE-1/Prox-1 E17.5 Expression is in lymphatic endothelial cells.
Specimen 4B: embryonic day 17.5 (more )
Note: Triple labeled: green - Lyve1; blue - Prox1 (assay MGI:5558009); red - Pecam1 (assay MGI:5558013).
Structure Level Pattern Image Note
TS25: intestine Present Regionally restricted 4B Expression is in lymphatic endothelial cells.
TS25: lymphatic system Present Regionally restricted 4B Expression is in lymphatic endothelial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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